对液体-液体色谱的六甲基二氧化/乙基异酸/乙醇/水溶剂系统的评估
Kai Wang1, Simon Vlad Luca1, Mirjana Minceva1
1Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany.
Journal of chromatography. A
|March 18, 2025
概括
研究人员开发了一种更绿色的溶剂系统,即六甲基二氧化/乙烯二氧化/乙醇/水,作为液体-液体色谱 (LLC) 的更安全替代品. 这种新系统的分离性能与传统的有毒溶剂系统相提并论,促进化学分离的环境安全.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
背景情况:
- 传统的液体-液体色谱 (LLC) 溶剂系统,如ARIZONA和HEMWat,使用有毒的,以石油为基础的溶剂,对人类健康和环境构成风险.
- 对于LLC应用,开发更安全,更环保的溶剂替代品至关重要.
研究的目的:
- 综合评估一种新型的双相溶剂系统,即甲基二氧化/乙基异酸盐/乙醇/水 (HMDSO/EIB/乙醇/水),作为LLC.的更绿色替代品.
- 将HMDSO/EIB/乙醇/水系统的热力学和物理性能以及分离性能与传统的HEMWat系统进行比较.
主要方法:
- 评估HMDSO/EIB/乙醇/水系统的热力学和物理性能 (密度差异,粘度,沉时间).
- 确定12个测试化合物的分布常数 (Ki) 在HMDSO/EIB/乙醇/水和HEMWat系统的16种组合中.
- 使用COSMO-RS进行Ki值的预测建模和使用逆流色谱和离心分区色谱进行实验验证.
主要成果:
- HMDSO/EIB/乙醇/水系统表现出相似的相密度差异,但比HEMWat系统具有更高的粘度和更长的沉降时间.
- 在两个溶剂系统之间确定了产生类似分布常数 (Ki) 的系统组成.
- COSMO-RS准确地预测了Ki值,促进了选择最佳的HMDSO/EIB/乙醇/水组成.
- 反流和离心分区染色学实验表明,在两种系统中,选定的化合物具有可比的分离行为.
结论:
- 该HMDSO/EIB/乙醇/水系统是一个可行的,更安全的替代传统有毒溶剂系统,如HEMWat和LLC的ARIZONA.
- 科斯莫-RS的预测能力可以显著简化识别适合特定分离的更绿色溶剂系统组合的过程.
- 这项研究有助于推进染色学和自然产品隔离的可持续实践.
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