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使用PacBio HiFi读取和Hi-C技术组装Xenocypris davidi的染色体规模

Tiezhu Yang^1,2, Liangjie Zhao^1,2, Chaoqun Su^1,2

¹College of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, 464000, China.

Scientific data

|March 19, 2025

概括

研究人员已经创建了中国水生生态系统中关键鱼类物种Xenocypris davidi的第一个染色体级基因组组. 这种基因组资源有助于理解其适应性,并支持遗传改进工作.

科学领域:

水生生态学水生生态学
基因组学就是基因组学.
动物学动物学

背景情况:

在中国的水生生态系统中,Xenocypris davidi在生态和经济上都很重要.
对X. davidi的有限基因组资源阻碍了对其适应和遗传改进的研究.

研究的目的:

为了产生第一个染色体水平的基因组组合Xenocypris davidi.
为未来的研究和物种的遗传增强提供基础的基因组资源.

主要方法:

使用了PacBio长读数,Illumina短读数和Hi-C测序数据.
组装了一个1.05Gb的基因组,用一个33.99Mb的N50支架.
将95.12%的基因组固定在24个伪染色体上.

主要成果:

成功生成了Xenocypris davidi的高质量的染色体级基因组组.
确定了27,360个编码蛋白质的基因,其中26672个功能性注释.
汇编为了解物种遗传学提供了一个强大的资源.

结论:

在Xenocypris davidi的染色体级基因组组合是一个显著的进步.
该资源将促进对进化适应和遗传改进策略的研究.
它支持对X. davidi. 农学特征的分子基础的探索.

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Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.