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代谢学策略用于诊断尿路感染

Carly C Y Chan¹, Daniel B Gregson^2,3, Spencer D Wildman¹

¹Alberta Centre for Advanced Diagnostics, Department of Biological Science, University of Calgary, Calgary, AB, Canada.

Nature communications

|March 19, 2025

概括

代谢学家现在可以使用两个关键代谢物,阿格马丁和N6-甲基亚丁氨酸来检测尿路感染 (UTI). 这种快速,准确的诊断方法显示出临床应用的前景.

科学领域:

生物化学生物化学
临床诊断临床诊断临床诊断
微生物学微生物学

背景情况:

代谢学是研究代谢表型的强大工具,但尚未成为临床诊断的标准.
尿路感染 (UTI) 是由于尿液中缩的微生物副产品而导致代谢的机会.
识别特定的尿路生物标志物可以实现更快的尿路感染诊断.

研究的目的:

研究代谢学在诊断尿路感染方面的潜力.
确定特定的代谢物,可以作为尿路感染的准确生物标志物.
开发一种用于检测尿路感染的快速诊断试验.

主要方法:

对怀疑尿路感染的患者的临床尿样进行了非向的代谢学查.
确定了阿格马丁和N6-甲基亚丁因作为培养阳性尿路感染的预测代谢物.
开发并验证了一种快速液体染色学-质谱学 (LC-MS) 试验,用于量化这些代谢物.

主要成果:

阿格马丁和N6-甲基亚丁因被确定为尿路感染的预测生物标志物.
开发的LC-MS试验准确地确定了由各种细菌物种引起的尿路感染 (超过90%的感染).
鉴定出的代谢物在两个大型失明患者队伍 (1629个样本) 中表现出强大的预测性能.

结论:

使用阿格马丁和N6-甲基亚丁的代谢药物显示出对尿路感染的快速临床诊断具有重大潜力.
这种方法为检测尿路感染提供了比传统的尿培养方法更快的替代方案.
这些发现支持将代谢学纳入传染病的常规临床诊断.

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