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Updated: May 21, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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Published on: March 16, 2017

黄血球Hmp和氧化降解酶通过限制低性NO信号,限制Bet-Hedging化剂Paracoccus denitrificans的初始nir表达,从而减少了化剂的NO信号

Ricarda Kellermann¹, Santosh Kumar², Andrew J Gates³

¹Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.

Environmental microbiology

|March 19, 2025

概括

血球蛋白Hmp和Nor调节氧化 (NO) 吸收,影响Paracoccus denitrificans中酸盐减少酶 (nirS) 的表达. 这种NO吸收在无氧呼吸过渡期间影响了对冲策略.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

氧化 (NO) 是细菌无氧呼吸中的关键中间体和信号分子.
NO调节化物 (Nir),氧化 (Nor) 和氧化 (N2OR) 的基因减少酶.
在Paracoccus denitrificans中,对冲策略可能涉及NO信号动态.

研究的目的:

研究一氧化 (NO) 清理系统在调节基因表达中的作用.
要了解关于NO信号的Paracoccus denitrificans的投注对冲策略.
为了确定无氧呼吸过程中nir诱导的特定触发因素.

主要方法:

利用一个带有mCherry-nirS融合的Paracoccus denitrificans菌株来监测nir表达.
在有氧条件下研究了Hmp黄血球蛋白在NO吸收中的功能.
检查了删除norCB对NO水平和投注对冲行为的影响.
同培养野生型和突变菌株,以评估NO介导的调节相互作用.

主要成果:

黄血球Hmp在有氧培养物中有效地清除NO,并由NsrR型抑制剂调节.
Hmp显著降低了最初的nirS表达,显示出负面的调节效应.
删除norCB取消了投注对冲,但NO升高并没有消除野生类型细胞中的投注对冲.
通过Hmp的NO清理,Nor在调节nirS表达方面发挥着关键作用.

结论:

和Nor对于通过NO清理调节nirS表达至关重要.
尼尔的诱导很可能是由细胞内NO衍生物引发的,而不是NO本身.
这些发现揭示了管理过渡到无氧呼吸的监管网络.

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