相关实验视频

Updated: May 21, 2025

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

西姆瓦斯塔丁调节了从上乳头分离出来的干细胞中的骨质分化

Paak Rewthamrongsris¹, Suphalak Phothichailert¹, Uraiwan Chokechanachaisakul²

¹Center of Excellence for Dental Stem Cell Biology and Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

BMC oral health

|March 19, 2025

概括

西姆瓦斯塔丁通过调节关键基因并促进矿物沉积,增强了来自上皮细胞 (SCAPs) 的干细胞的骨质分化. 这表明simvastatin.

科学领域:

生物材料科学生物材料科学
干细胞生物学干细胞生物学
恢复性牙科恢复性牙科

背景情况:

已知simvastatin会影响干细胞功能,包括维护和分化.
牙上皮囊 (SCAP) 的干细胞对于牙组织再生至关重要.

研究的目的:

调查simvastatin对SCAPs体外骨质原体分化的影响.
为了阐明simvastatin对SCAP骨质生成的作用背后的分子机制.

主要方法:

SCAPs被隔离并进行了表征.
细胞接受了不同度的simvastatin (100-1,000 nM) 的治疗.
通过基因表达 (RNA测序,qPCR),性酸酶活性和矿化试验 (Alizarin Red S,Von Kossa染色) 来评估骨质分化.

主要成果:

西姆瓦斯塔丁在骨质生路径 (例如,TGF-β,FoxO,MAPK) 上调的基因和下调的增殖/亡基因.
矿物沉积和骨质标记基因表达 (OSX,DMP1,DSPP,OCN) 剂量取决于增加.
抑制TGF-β受体部分阻断了simvastatin的作用.

结论:

西姆瓦斯塔丁促进SCAP中的骨质分化,可能通过TGF-β信号传递.
西姆瓦斯塔丁作为牙脉再生的辅助疗法在重要脉治疗中显示出前景.

关键词:

细胞迁移细胞迁移细胞的增殖细胞的增殖.细胞活力细胞活力西姆瓦斯塔丁是一种来自顶端乳头的干细胞.

更多相关视频

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...