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相关概念视频

RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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相关实验视频

Updated: May 21, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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m6A2Circ:一个全面的数据库来解码m6A修饰和循环RNA之间的调节关系.

Yongtian Li1,2, Bianli Gu3, Lixia Ma3

  • 1Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.

Computational and structural biotechnology journal
|March 19, 2025
PubMed
概括

本研究介绍了m6A2Circ,这是一个详细介绍圆形RNA (circRNAs) 中N6-甲基氨酸 (m6A) 修饰的数据库. 它提供了关于m6A-circRNA相互作用的广泛数据,以推进该领域的研究.

关键词:
这是循环RNARNA.数据库数据库数据库是一个数据库.这是一种N6-甲基氨酸.

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相关实验视频

Last Updated: May 21, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Identification of Circular RNAs using RNA Sequencing
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 循环RNAs (circRNAs) 是由mRNA前后拼接形成的非编码RNA.
  • N6-甲基氨酸 (m6A) 修饰越来越多地被认为是其在circRNA代谢中的调节作用.
  • 缺乏对m6A-circRNA相互作用的全面资源.

研究的目的:

  • 开发一个专门的数据库,m6A2Circ,用于探索circRNAs中的m6A修饰.
  • 系统地编目m6A-circRNA调节相互作用.
  • 促进研究circRNA功能和疾病相关性.

主要方法:

  • 来自人类和小鼠组织的m6A-circRNA关联的编译.
  • 基于实验和高通量测序数据的关联分类.
  • 开发一个可以通过网络访问的数据库 (http://m6a2circ.canceromics.org/).

主要成果:

  • 在m6A2Circ数据库中包含198804个m6A-circRNA关联.
  • 协会是由四个层次的证据支持的.
  • 该数据库为circRNA研究提供了广泛的注释.

结论:

  • m6A2Circ解决了对circRNAs中的m6A修饰的全面资源的需求.
  • 该数据库支持对m6A-circRNA交叉的功能和疾病影响的研究.
  • m6A2Circ旨在促进RNA表观遗传学和非编码RNA生物学方面的新发现.