相关实验视频
Updated: May 5, 2026
09:16
Assessment of Kidney Function in Mouse Models of Glomerular Disease
Published on: June 30, 2018
17.5K
糖尿病病:m6A修饰作为疾病进展和亚型分类的标记
Wenzhe Li1, Gaosi Xu1, Manna Li2
1The Second Clinical Medical College of Nanchang University, Nanchang, China.
Frontiers in medicine
|March 19, 2025
概括
这项研究揭示了在糖尿病病 (DKD) 进展中明显的N6-甲基氨酸 (m6A) 修饰模式. 识别特定的m6A调节剂和亚型为早期诊断和向治疗提供了潜力.
科学领域:
- 分子生物学分子生物学
- 腎臟病學 (nephrology) 是一種醫學專業.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 糖尿病病 (DKD) 是糖尿病的一个主要并发症,其特征是复杂的分子变化.
- N6-甲基氨酸 (m6A) 修饰是最丰富的内部mRNA修饰,在包括病在内的各种生物过程中起着至关重要的作用.
- 了解m6A调节器在DKD进展中的作用对于开发新型治疗策略至关重要.
研究的目的:
- 研究糖尿病病 (DKD) 进展过程中m6A调节者的作用和表达模式.
- 识别潜在的基于m6A的生物标志物用于早期DKD检测,并将DKD分类为不同的分子亚型.
- 阐明与不同m6A改性DKD亚型相关的免疫特征和分子机制.
主要方法:
- 对外围血液单核细胞,脏组织,质细胞和DKD患者和对照者的管间样本中的m6A调节者的表达分析.
- 生物信息分析包括CIBERSORT,用于免疫分析的ssGSEA,用于生物标志物模型开发的Logistic-LASSO回归,用于亚型分类的共识聚类,用于途径丰富的GSVA,用于标志物基因识别的WGCNA和PPI网络.
- 利用Nephroseq V5工具将已识别的标记基因与膜过率 (GFR) 等功能参数相关联.
主要成果:
- 在多种组织类型的DKD患者和对照人群之间观察到m6A调节器表达的显著差异,在不同的DKD阶段观察到不同的免疫特征.
- 包括YTHDC1,METTL3和ALKBH5在内的预测模型在识别早期DKD时表现出有效性.
- 根据m6A调节器的表达,DKD被分为两种亚型:亚型1 (肌生成,原,细胞因子相互作用) 和亚型2 (蛋白质分泌,增殖,亡,TGFβ,PI3K/AKT/mTOR通路). AXIN1和GOLGA4成为与GFR相关的潜在生物标志物.
结论:
- m6A 修改显著影响免疫格局和基底的分子机制 DKD 进展.
- 确定的m6A调节器模型和DKD亚型为疾病异质性和潜在的治疗点提供了新的见解.
- 基于m6A修饰模式的有针对性的干预措施可以提高DKD患者的治疗疗效.
更多相关视频
07:11A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
Published on: September 11, 2020
1.4K
07:15Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
303
相关概念视频
01:24Nephrotic Syndrome I : Introduction
1.2K
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
1.2K
01:25Chronic Kidney Disease I: Introduction
1.4K
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
1.4K
01:24Chronic Kidney Disease II: Clinical Manifestations
1.1K
Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
1.1K
01:28Chronic Kidney Disease III: Interprofessional Care
706
Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
706
01:27Diabetic Retinopathy
65
DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
65
01:28Diabetic Nephropathy
49
Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration...
49