确定最佳抗氧化策略:通过LC-HRMS和计算建模揭示的Cistus villosusL.提取物中提取方法的比较分析
Hicham Bouakkaz1, Ebru Erol2, Khedidja Benarous1
1Fundamental Sciences Laboratory, Amar Telidji University, Laghouat, Algeria.
Turkish journal of biology = Turk biyoloji dergisi
|March 19, 2025
概括
一种茶叶草的Cistus villosus含有强大的抗氧化剂,可以抵御氧化应激. 它的乙酸乙烯提取物表现出显著的抗氧化活性,在DPPH测试中超过了α-托科菲罗尔.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 自然产品 自然产品
背景情况:
- Cistus villosus L. 是一种传统的茶叶草,具有已知的抗氧化特性.
- 氧化压力是一个重要的现代健康问题,与各种疾病有关.
- 天然化合物为对抗氧化应激提供了一个有希望的途径.
研究的目的:
- 为了评估Cistus villosus L.乙烯酸提取物的抗氧化潜力.
- 为了识别和描述提取物中的抗氧化化合物.
- 通过分子对接和计算研究来研究抗氧化剂作用的机制.
主要方法:
- 进行了抗氧化剂测试 (例如DPPH激素清除测试).
- 液体染色学高分辨率质谱法 (LC-HRMS) 用于化合物识别.
- 进行了分子对接,ADMET和PASS (预测系统系统) 分析.
主要成果:
- 乙酸乙烯提取物表现出强烈的抗氧化活性,在DPPH试验中IC50值为5.30 mg/L,表现优于α-托哥菲醇.
- 确定了22种化合物,这些化合物具有已记录的抗氧化能力.
- 分子对接和PASS研究表明,这些化合物通过向特定途径来抑制氧化剂形成的有效性.
结论:
- Cistus villosus L. 提取物是有效抗氧化剂的丰富来源.
- 这些已识别的化合物显示出在治疗氧化应激管理中的治疗应用潜力.
- 这种草代表了开发基于抗氧化剂的健康策略的宝贵自然资源.
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