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隔离和识别导致果中收获后黑色腐烂的Alternaria alstroemeriae,并使用含有黄素的纳米脂质体对其进行控制

Jie Li¹, Zuyun Zhang¹, Ping Yang¹

¹College of Biological and Agricultural Sciences, Honghe University, Mengzi, China.

Frontiers in microbiology

|March 19, 2025

概括

含有黄素的纳米脂质体通过增强植物防御酶和水果质量,有效地对抗黑腐烂. 这种新的方法对可持续农业和保护果生产免受真菌病原体的影响具有前景.

科学领域:

植物病理学植物病理学
农业科学农业科学
纳米技术纳米技术

背景情况:

由*Alternaria alstroemeriae*引起的黑腐烂对类产业的产量产生了重大影响.
在云南省鉴定腐病原体对于有效的疾病管理至关重要.
开发新的,可持续的策略来控制收获后类疾病是必不可少的.

研究的目的:

确定在云南省导致类水果腐烂的致病真菌.
合成黄素载入的纳米脂质体作为一种潜在的管理策略.
评估黄素载荷纳米脂质体对*Alternaria alstroemeriae*的疗效及其对类植物生理学和水果质量的影响.

主要方法:

用形态学和rDNA ITS序列分析分离,净化和识别致病真菌.
合成含有黄素的纳米脂质体.
在体外和体内实验,以评估纳米脂质体对类植物的抗真菌活性和生理效应.

主要成果:

确定了*A. alstroemeriae*,*Rhizopus arrhizus*,*Aspergillus flavus*和*Penicillium digitatum*作为类腐烂的致病原体.
含有黄素的纳米脂质体表现出10μmol/L的最小杀菌度 (MIC) 对抗A. alstroemeriae*的菌生长.
在体内治疗显著降低了黑色腐烂的发生率,增强了类防御酶活动 (PPO,APX,POD,PAL,GR,CAT),并改善了水果质量参数 (TSS,TA,,黄).

结论:

含有黄素的纳米脂质体有效地抑制了A. alstroemeriae,并赋予了对类黑色腐烂的抵抗力.
纳米脂质体作为植物诱导剂,调节生理反应并提高水果质量.
载有黄素的纳米脂质体代表了一种有希望的,可持续的方法来管理类果收获后的疾病.

关键词:

这是一种抗真菌药物.含有黄素的纳米脂体.形态分析分析形态分析病原性病原性病原性收获后疾病收获后疾病

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