相关实验视频

Updated: May 21, 2025

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
06:21

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

6.4K

准备使用的EGF变种的重组表达,配备单个结合部位用于点击化学

Melanie Krass1, Meike Kolster2, José Ignacio Valenzuela3

  • 1Institute of Diagnostic Laboratory Medicine, Clinical Chemistry and Pathobiochemistry Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin Berlin Germany.

Engineering in life sciences
|March 19, 2025
PubMed
概括

研究人员设计了一种用于向癌症治疗的新型K-EGF变体,通过克服结合挑战,实现精确的药物输送. 这种工程表皮生长因子 (EGF) 促进了向部分和药物分子的均合.

关键词:
通过点击化学.皮肤表皮生长因子连带变体的变体单一的结合部位是单一的结合部位有针对性的治疗.

更多相关视频

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells12:09

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

10.7K
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.4K

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.1K