微弱有机酸效应的细菌光驱的质子Rhodopsin
Zikun Lyu1, Shunki Takaramoto1, Keiichi Inoue1
1The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba 277-8581, Japan.
The journal of physical chemistry. B
|March 19, 2025
概括
微弱有机酸 (WOA) 效应增强了细菌罗多素中的质子,如PspR来自Pseudomonas putida. 细胞外WOA加速质子运输,揭示了细菌与植物相互作用的新见解.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 微生物学 微生物学
背景情况:
- 微生物罗多普辛是光驱生物机器.
- 向外出质子 (H+),罗多素通过膜传输H+.
- 微弱的有机酸 (WOA) 效应增强了真菌罗多普辛中的H+.
研究的目的:
- 为了研究WOA效应在细菌外向H+Rhodopsins.
- 为了确定细胞外弱有机酸是否影响来自Pseudomonas putida的PSPR活性.
主要方法:
- 在纯化的PSPR蛋白质上进行过渡吸收测量.
- 在光循环过程中分析H+吸收和释放动力学.
- 评估乙酸和英多尔-3-乙酸 (IAA) 对PSPR活性的影响.
主要成果:
- 细胞外酸和IAA增强了细菌PSPR的H+活动.
- WOAs加速细胞质H+吸收和细胞外H+释放.
- 细胞质乙烯酸抑制PSPR活性,对IAA的影响各不相同.
结论:
- WOA效应存在于细菌向外H+的Rhodopsins中.
- 细胞外WOA调节了PSPR的光循环和H+运输.
- 这些发现为细菌罗多普辛在植物相互作用中的生理作用提供了新的视角.
更多相关视频
10:03Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
17.9K
10:39Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures
Published on: April 14, 2023
2.4K
相关概念视频
Channel Rhodopsins
2.5K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.5K
Photoreceptors and Visual Pathways
5.5K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
5.5K
G-Protein Gated Ion Channels
4.5K
GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
Sensory...
4.5K
The Photochemical Reaction Center
4.0K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.0K
