一个编码在核中的类似胺的蛋白质控制了trypanosomatidAngomonas deanei中的内生生体分裂
Anay K Maurya1, Lena Kröninger1, Georg Ehret1
1Institute of Microbial Cell Biology, Department of Biology, Heinrich Heine University Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany.
Science advances
|March 19, 2025
概括
在Angomonas deanei中,宿主蛋白 (ETP9) 对于同步细胞分裂与其细菌内共生体至关重要. 这种蛋白质补偿了丢失的细菌分裂基因,揭示了双源分裂机制.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 交生研究 交生研究
背景情况:
- 安哥马纳斯deanei寄存一个单一的β-蛋白质细菌内共生体,与同步的宿主和内共生体细胞周期.
- 这种复杂的宿主-内生体细胞周期同步的分子基础在很大程度上是未知的.
- 一个先前已识别的核编码的胺类蛋白质,ETP9,定位到A. deanei.的内生生体分裂部位.
研究的目的:
- 研究A. deanei及其β-蛋白质细菌内共生体之间的同步细胞分裂背后的分子机制.
- 阐明宿主编码ETP9蛋白在内生生体分裂中的作用.
- 了解内生生体分裂机器的进化适应.
主要方法:
- 在 Strigomonadinae 子家族中进行比较基因组学分析.
- 对ETP9.9的细胞周期依赖的亚细胞局部化研究.
- 在共生和非共生A. deanei菌株中使用敲除方法对ETP9的功能分析.
主要成果:
- Strigomonadinae亚家族中的内共生动物已经失去了独立形成分裂隔膜的能力.
- ETP9的局部化取决于细胞周期,并与细菌FtsZ蛋白在分裂部位的积累相吻合.
- 对于共生A. deanei来说,ETP9至关重要,但对于缺乏内共生A. deanei的非共生菌株来说,ETP9至关重要.
- 在共生A. deanei中,对ETP9的破坏导致有线状,分裂受损的内共生体.
结论:
- 在A. deanei. 中,一种具有双重遗传起源的新型内生生体分裂系统已经进化.
- 一种来自宿主的蛋白质 (ETP9) 已经被新功能化,以弥补细菌分裂基因的损失.
- 这项研究揭示了宿主-内共生生物共同进化的独特例子,影响了像分裂这样的基本细胞过程.
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