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阐明牛肉汁中的Listeria monocytogenes生物膜形成过程,微观结构和功能基因表达

Yangtai Liu¹, Lili Hu¹, Zhuosi Li¹

¹University of Shanghai for Science and Technology, 516 Jun Gong Rd., Shanghai 200093, China.

International journal of food microbiology

|March 19, 2025

概括

牛肉汁在肉类加工环境中增强了Listeria monocytogenes生物膜的形成. 这项研究揭示了牛肉汁如何影响生物膜结构和基因表达,为控制污染提供了洞察力.

科学领域:

微生物学微生物学
食品安全食品安全
细菌病原体的产生

背景情况:

李斯特菌单细胞菌生物膜是食品工业中交叉污染的重要来源,特别是与生牛肉有关.
特定菌株,Listeria monocytogenes序列类型9 (ST9) 和ST8,经常在肉类和加工设施中发现.
了解相关食物矩阵中的生物膜形成对于开发有效的控制策略至关重要.

研究的目的:

研究牛肉汁对Listeria monocytogenes ST9和ST8菌株生物膜形成的影响.
分析生物膜微观结构和模拟牛肉汁中的生物膜形成过程与实验室介质对比.
在不同的培养条件下检查关键生物膜功能基因的表达.

主要方法:

在牛肉汁和豆中通过Listeria monocytogenes ST9和ST8形成生物膜的比较与酵母提取物 (TSB-YE).
使用扫描电子显微镜分析生物膜微结构.
使用物流模型建模生物膜形成动力学,并量化基因表达 (agrA,inlB,actA).

主要成果:

牛汁促进了密集的,三维的Listeria monocytogenes生物膜,与TSB-YE中的单层结构不同.
ST9菌株的生物膜形成比ST8.8菌株更大. 牛肉汁增加了最大特定生物膜形成率 (μmax),但降低了最大生物膜数量 (Ymax).
观察到基因表达的显著差异:在牛肉汁中,agrA是下调的,而inlB和actA是上调的,特别是对于ST8.8.

结论:

牛肉汁对Listeria monocytogenes生物膜的发展起到有利的媒介作用,这可能是由于其作为表面调节剂的成分.
这些发现突出了生物膜形成的菌株特异性以及食物矩阵对细菌行为的影响.
这项研究为控制肉类加工环境中的Listeria monocytogenes污染提供了宝贵的见解.

关键词:

食品安全食品安全营养环境营养环境预测模型是一个预测模型.扫描电子显微镜扫描电子显微镜

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