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网络分析和实验验证分析揭示了psoralen改善葡萄皮质激素诱导的增长迟缓的机制

Wenxiang Zeng¹, Ying Zhao², Qingyu Tu¹

¹The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China; Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou 311200, China.

Journal of pharmaceutical and biomedical analysis

|March 19, 2025

概括

松素 (PSO) 可以治疗儿童的葡萄糖皮质激素诱导的增长迟缓 (GIGR). 这项研究发现,PSO通过激活PI3K/AKT通路来增强生长板冠状细胞的增殖和骨生长,从而提供了潜在的治疗策略.

科学领域:

药理学药理学是指药理学的学科.
内分泌学在内分泌学.
细胞生物学细胞生物学

背景情况:

葡萄糖皮质激素 (GCs) 是广泛使用的药物,具有显著的副作用,包括儿童患者的生长迟缓.
GC诱导的生长迟缓 (GIGR) 通过抑制生长板体细胞中的内分泌骨化,对骨发育产生负面影响.
松烯 (PSO) 已显示出在保护软骨平衡和增强软骨细胞增殖方面的潜力,但其对抗GIGR的机制尚未完全理解.

研究的目的:

调查psoralen (PSO) 在减轻葡萄糖皮质类药物诱导的增长迟缓 (GIGR) 的治疗潜力.
阐明PSO影响GC受损生长板冠状细胞的潜在分子机制.

主要方法:

网络药理学和分子对接被用来确定PSO的潜在治疗点.
在大鼠模型和体外细胞培养中进行的实验验证实,评估了PSO对生长板冠状细胞的影响.
西方涂抹和免疫光被用于分析蛋白质表达和信号通路激活.

主要成果:

与对照组相比,PSO治疗显著增加了大鼠的股骨长度和生长板大小.
实验室研究表明,PSO增强了生长板冠状细胞的增殖,并维持了细胞平衡.
PSO激活了PI3K/AKT信号通路,增加了与软骨相关的蛋白质表达,并降低了apoptotic蛋白质水平.

结论:

松烯 (PSO) 显示出对GC诱导的生长板胆固醇细胞损伤的保护作用.
通过激活PI3K/AKT信号通路,PSO促进骨生长和软骨细胞的增殖.
PSO代表了一个有前途的治疗候选人,用于管理GC诱导的生长迟缓 (GIGR).

关键词:

葡萄糖皮质类皮质类药物增长减缓增长减缓分子对接是分子对接.网络药理学网络药理学通过PI3K/AKT路径.这是一种Psoralen.

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