从甘残留物中使用金属改性生物炭增强暗发酵的生产:优化,表征和代谢分析
Nantharat Wongfaed1, Sureewan Sittijunda2, Sompong O-Thong3
1Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen, 40002, Thailand.
Journal of environmental management
|March 19, 2025
概括
金属改性甘生物炭显著提高了黑色发酵 (HP) 的生产,提高了产量超过58%. 优化生物炭和金属含量是有效生产生物的关键.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 环境科学 环境科学
背景情况:
- 暗发酵生产 (HP) 是一个有前途的生物能源技术.
- 从农业废物中提取的生物炭可以成为微生物过程的可持续基质.
- 生物炭的金属修饰可以增强其催化和吸附性质.
研究的目的:
- 调查使用Fe和Ni修改的甘包 (SB) 和甘叶 (SL) 生物炭来增强暗发酵的生产.
- 为了优化金属改性生物炭的剂量,以获得最大的产量.
- 阐明在增强生产的基础上存在的微生物和代谢机制.
主要方法:
- 甘包和叶子被转化为生物炭,并用铁 (Fe) 和 (Ni) 进行修改.
- 采用了Box-Behnken设计,以优化用于生产的生物炭剂量.
- 进行了微生物群体分析和代谢途径分析.
主要成果:
- 确定了SB生物炭的最佳剂量为6.09g/L,SL+Fe生物炭的最佳剂量为5.38g/L,SL+Ni生物炭的最佳剂量为7.66g/L.
- 获得了108.77毫升H2 / g葡萄糖的最大产量,与对照组相比增加了58.77%.
- 金属修饰增强了生物炭的表面特性,丰富了特定的产生的细菌 (Clostridium sensu stricto 1, Paraclostridium sp. ),并改善了葡萄糖代谢.
结论:
- 甘衍生生物炭,特别是当与Fe和Ni进行修改时,是暗发酵生产的有效增强剂.
- 优化金属度至关重要,以最大限度地提高产量,避免潜在的抑制作用.
- 这种方法为从农业废物中生产生物提供了一个可持续的途径.
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