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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.1K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

95.4K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
95.4K

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相关实验视频

Updated: May 21, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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用于基因检测的电化学活性DNA配体:现在和未来

Shigeori Takenaka1, Shinobu Sato2

  • 1Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu, 804 8550, Japan. shige@che.kyutech.ac.jp.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
|March 20, 2025
PubMed
概括

电化学基因传感使用DNA连接物进行准确的诊断. 铁甲二胺 (FNDs) 能够敏感地检测癌症标记物和病毒RNA,为PCR提供可靠的替代方案.

科学领域:

  • 电化学 电化学 电化学
  • 分子诊断学 分子诊断
  • 生物传感器是一种生物传感器.

背景情况:

  • 电化学基因传感正在成为诊断芯片的强大工具.
  • 包括金属复合体,甲蓝和铁甲二胺 (FND) 在内的DNA配体对于这些传感方法至关重要.

研究的目的:

  • 审查基于电化学活性DNA连接体的电化学基因传感方法.
  • 突出这些方法在疾病诊断和遗传分析中的应用.

主要方法:

  • 将DNA探头固定在电极表面上.
  • 混合化反应与目标DNA片段.
  • 电化学检测使用像FND,甲基蓝和FNC这样的DNA配体.

主要成果:

  • 通过FND检测hTERT基因甲基化的成功癌症诊断.
  • 使用乙烯蓝 (关闭信号) 和FNC (启动信号) 电化学检测PCR产品.
  • 使用FND和G-四倍体 (G4) 相互作用,电化学检测端粒酶活性和新冠病毒.

结论:

  • 用DNA配体进行电化学基因传感为诊断提供了可靠而敏感的平台.

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Last Updated: May 21, 2025

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  • 通过G4结构,FND在检测癌症标记物和病毒RNA方面表现出多功能性.
  • 这项技术有望开发快速,非侵入性诊断测试,包括点的护理应用.