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人类带衍生中干细胞改善了D-银糖诱导的老年小鼠的胸腺和脏功能

Jianwei Xu^1,2, Li Dong³, Xiaofen Xie²

¹Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, Guiyang, China.

Scientific reports

|March 20, 2025

概括

带介质干细胞 (UC-MSCs) 可以逆转免疫器官的衰老,如胸腺和脏. 这种治疗改善了免疫功能和肠道微生物群平衡,提供了一个延迟免疫衰老的策略.

科学领域:

免疫学免疫学免疫学
老年学是一门学科.
干细胞生物学干细胞生物学

背景情况:

免疫衰老,以免疫器官结构和功能恶化为特征,是衰老的标志.
在实验研究中,D-银糖的使用是诱导免疫衰老的常见模式.
保持免疫平衡对于老化期间的整体健康至关重要.

研究的目的:

研究带介质干细胞 (UC-MSCs) 在缓解D-银糖诱导的免疫衰老方面的疗效.
评估UC-MSCs对老化免疫器官 (胸腺和脏) 的结构和功能完整性的影响.
评估UC-MSCs在调节肠道微生物群组成中的作用及其与免疫衰老的关系.

主要方法:

给D-银糖诱导的老化小鼠进行了UC-MSC.
进行了胸腺和脏的组织学分析,评估了缩和衰老相关的β-galactosidase (SA-β-gal) 阳性细胞.
对与衰老相关的标记物 (p16,p53,p21,RB) 进行基因表达分析.
测量了抗氧化酶活性 (CAT,SOD,GSH-Px) 和甲 (MDA),评估了Nrf2/HO-1通路的激活.
用测序技术分析了肠道微生物群的组成.

主要成果:

治疗UC-MSCs显著延迟了胆髓和脏缩,并减少了SA-β-gal阳性细胞.
UC-MSCs降低了与衰老相关的基因的表达 (p16,p53,p21,RB).
UC-MSCs增强了抗氧化酶活性,增加了抗氧化剂水平,降低了MDA,并激活了Nrf2/HO-1通路.
治疗UC-MSC恢复了肠道微生物组合的平衡.

结论:

通过保护免疫器官结构和功能,UC-MSCs有效地减轻D-银糖诱导的免疫衰老.
UC-MSCs通过抗氧化机制和调节与衰老相关的基因表达产生保护作用.
UC-MSCs促进肠道微生物群平衡,有助于延迟免疫衰老.
UC-MSCs代表了一种有前途的治疗策略,用于打击与年龄相关的免疫衰退.

关键词:

衰老的衰老衰老的衰老我们的肠道微生物群.人类带衍生中介细胞干细胞.Nrf2 / HO-1 的一个.甲状腺和脏都是如此.

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