相关实验视频

Updated: May 21, 2025

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

基于转录基因组的采矿,用于在虫 (Fusarium fujikuroi) 中高生产吉伯林GA3的强促剂

Qi Guo¹, Yue-Feng Zhong¹, Xin-Yu Chen¹

¹School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing 210023, People's Republic of China.

Journal of agricultural and food chemistry

|March 20, 2025

概括

研究人员在Fusarium fujikuroi中发现了一种强大的构成性促进剂,用于增强吉伯雷林GA3的产生. 这一发现促进了代谢工程的努力,导致了这一重要的植物生长调节器的显著提高产量.

科学领域:

微生物学微生物学
生物技术是生物技术.
植物科学植物科学

背景情况:

虫 (Fusarium fujikuroi) 是工业性吉伯雷林GA3生产的关键宿主.
在F. fujikuroi中精确的代谢流量调节是具有挑战性的,因为缺乏已识别的内源性促进剂.

研究的目的:

挖掘并为F. fujikuroi建立一个促进者图书馆.
为了识别和表征强大的构成性促进器的代谢工程.
通过新陈代谢流量调节来增强吉伯雷林GA3的过度产量.

主要方法:

转录组测序被用来挖掘潜在的促进体.
建立了一个由20位潜在发起人组成的图书馆.
用β-葡萄糖酶 (GUS) 记者基因测试来评估促进体活性.
促进物P被用来设计F. fujikuroi对GA3的过度生产.

主要成果:

一个新的促进者图书馆为F. fujikuroi成功建造.
发起人P被确定为具有稳定的表达力的强有力的构成性发起人.
与对照组相比,GA3产量在摇瓶中增加了17.1% (0.89 g/L).
一种工程菌株 (FF-2) 在5L生物反应器中实现了2.38g/L的GA3产量.

结论:

这项研究为F. fujikuroi.提供了宝贵的促进者资源.
使用已识别的促进剂进行的工程代谢流量调节显著增强了GA3的产生.
这项工作促进了F. fujikuroi的代谢工程,以改善工业应用.

关键词:

富萨里乌姆 (Fusarium) 是一个富士基罗伊 (fujikuroi) 的植物.吉伯雷林 GA3 是一种活动主办人活动主办人对转录组进行分析.这是一种β-Glucuronidase.

更多相关视频

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

相关概念视频

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...