在细菌分工中影响种姓比例的因素
Luis Alfredo Avitia Domínguez1, Zhengzhou Yu1, Varun Chopra1
1Institute of Biology, Leiden University, Leiden 2333 BE, The Netherlands.
概括
斑马菌 (Streptomyces coelicolor) 的殖民地调整了它们的专门细胞比例,突变频率随着年龄的增长和对竞争的反应而增加. 这种分工是塑性的,适应环境的线索.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 细菌遗传学 细菌遗传学
背景情况:
- 菌群Streptomyces coelicolor在生长/分泌和抗生素生产之间表现出分工.
- 这种专业化与抗生素过度生产细胞中昂贵的染色体缺失有关.
- 这些突变的空间和时间动态及其可塑性以前是未知的.
研究的目的:
- 研究Streptomyces coelicolor殖民地突变的空间分布和时间出现.
- 为了确定突变频率,代表专门的细胞比例,是否是表型塑性.
- 了解环境因素对这种分工的影响.
主要方法:
- 在各种条件下种植的Streptomyces coelicolor殖民地采样.
- 测量突变频率作为专门细胞比例的指标.
- 暴露于亚抑制性抗生素度和与其他杆菌物种的直接竞争.
主要成果:
- 突变频率随着殖民地的年龄和大小线性增加.
- 突变在殖民地中心不成比例地积累,与外围生长和分泌形成形成对比.
- 暴露于抗生素和与其他Streptomyces的竞争增加了突变频率和子生产.
结论:
- 菌株菌群中的突变频率是动态的,并且对竞争环境有反应.
- 染色体缺失是表型塑性,允许灵活调整专门细胞比例.
- 提供了对驱动微生物殖民地分工的内在和环境因素的见解.
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