相关实验视频

Updated: May 21, 2025

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

在肺腺癌中,TNFRSF21的丧失会诱导思普拉丁敏感性

Daien Zhou¹, Haoyang Yuan², Yiwei Hu³

¹Department of Thoracic Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an, Huai'an, 223001, China.

Oncology research

|March 20, 2025

概括

瘤坏死因子受体超级家族成员21 (TNFRSF21) 在肺腺癌 (LUAD) 中被上调,促进瘤细胞的增殖和耐药性. 下调TNFRSF21可能为肺癌化疗提供一个新的策略,特别是针对癌症干细胞 (CSCs).

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症治疗方法癌症治疗方法

背景情况:

肺腺癌 (LUAD) 存在治疗挑战,尽管已确定目标.
瘤亡因子受体超级家族成员21 (TNFRSF21) 与各种癌症有关.
这项研究调查了TNFRSF21在LUAD进展和治疗耐药性的特定作用.

研究的目的:

评估TNFRSF21表达在肺癌中的预后意义.
阐明TNFRSF21在LUAD细胞活力,增殖和化学抵抗中的功能作用.
探索TNFRSF21在LUAD中调节活性氧物种 (ROS) 和癌症干细胞 (CSC) 特性中的参与.

主要方法:

预测值使用GEPIA和Kaplan-Meier Plotter数据库进行评估.
用CCK8测定测量细胞活力;用RT-PCR测量基因表达.
瘤球形成和MitoSOX光试验评估了CSC类属性和ROS水平.

主要成果:

在LUAD组织和细胞系中,TNFRSF21的表达显著上调,包括对西斯丁 (DDP) 耐药的细胞.
丧失TNFRSF21抑制LUAD细胞生长,而其强制表达增强了增殖和DDP抵抗.
降低TNFRSF21的调节会增加ROS的产生,通过CD44和CD133调节抑制CSC类特征.

结论:

TNFRSF21在促进LUAD细胞增殖和化学抵抗方面发挥着至关重要的作用.
TNFRSF21调制影响LUAD中的ROS水平和CSC类属性.
TNFRSF21代表了肺癌化疗的潜在新型治疗标,特别是消除CSCs.

关键词:

癌症干细胞 (CSCs) 是一种癌症干细胞.西斯普拉丁的敏感性肺癌是一种肺癌.美国TNFRSF21

更多相关视频

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

Author Spotlight: Establishing a Murine Non-Small Cell Lung Cancer Model for Developing Nanoformulations of Anticancer Drugs

Author Spotlight: Establishing a Murine Non-Small Cell Lung Cancer Model for Developing Nanoformulations of Anticancer Drugs

Published on: May 10, 2024

相关概念视频

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...