提取,分析和抗氧化作用的棕脚多糖
Shiyang Zhou1,2,3, Wenbin Wang1, Jun Tan3
1Chongqing Chemical Industry Vocational College, Chongqing, 401228 China.
Food science and biotechnology
|March 20, 2025
概括
棕枝多糖体显示出显著的抗氧化作用,通过增强关键酶和减少损伤标志物,保护细胞免受氧化应激. 这种天然化合物在细胞和动物模型中显示出对抗氧化损伤的前景.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 氧化应激与各种疾病有关.
- 自然的多糖因其健康益处而受到探索.
- 棕树多糖 (PPP) 是生物活性化合物的潜在来源.
研究的目的:
- 描述PPP的结构性质.
- 评估PPP对过氧化 (H2O2) 诱导的氧化应激的抗氧化和细胞保护作用.
- 调查PPP的体外和体内疗效.
主要方法:
- 对PPP成分的结构分析 (拉姆诺,阿拉,银,葡萄糖).
- 在实验室对暴露于H2O2的RAW264.7细胞进行检测,以测量抗氧化酶 (CAT,SOD,GSH-Px) 和脂质过氧化 (MDA) 水平.
- 在小鼠体内研究以评估血清和肝脏抗氧化酶和MDA水平.
- 在H2O2受损细胞中分析氧化应激通路蛋白 (Nrf2,HO-1,NQO1).
主要成果:
- PPP主要由拉姆诺糖,阿拉伯糖,银糖和葡萄糖组成.
- 在体外,PPP显著增加了CAT,SOD和GSH-Px水平,同时降低了H2O2处理的RAW264.7细胞中的MDA,显示出剂量依赖的效果.
- 在体内,PPP提高了SOD和GSH-Px活性,并降低了小鼠血清和肝脏中的MDA含量.
- 在H2O2受损细胞中,PPP上调了Nrf2,HO-1和NQO1的表达,这表明Nrf2介导的抗氧化途径被激活了.
结论:
- 棕树多糖具有显著的抗氧化和细胞保护性质.
- 在细胞和动物模型中,PPP有效地减轻H2O2诱导的氧化应激.
- 这些发现表明PPP作为氧化压力相关条件的治疗剂的潜力.
相关概念视频
01:36Carbohydrate Metabolism
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...
01:10Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
01:10Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
01:19Overview of Carbohydrate Metabolism
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
01:26Biosynthesis of Polysaccharides
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...