昆虫基因病毒媒介RNAi在植物中控制虫的潜力
Baozheng Shi1, Haifang He1, Chenchen Zhao1
1College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.
Journal of agricultural and food chemistry
|March 20, 2025
概括
这项研究表明,病毒诱导的基因沉默 (VIGS) 可以通过表达针对重要基因的双链RNA (dsRNA) 来有效控制桃花. 结合VIGS和真菌Metarhizium anisopliae进一步增加了虫死亡率.
科学领域:
- 植物生物技术 植物生物技术
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
背景情况:
- 使用双链RNAs (dsRNAs) 的RNA干扰 (RNAi) 是昆虫害虫控制的一个有前途的策略.
- 病毒诱导的基因沉默 (VIGS) 能够在没有基因组改变的情况下实现dSRNA的植物表达.
- 三酶 (TRE) 和酸合成酶 (CHS) 是昆虫生理学的关键酶.
研究的目的:
- 评估针对三酶 (TRE) 和胆合成酶 (CHS) 的dSRNAs对桃花,Myzus persicae.的疗效.
- 调查VIGS用于植物媒介dSRNAs的表达,以控制M. persicae.
- 评估dsrna治疗和metarhizium anisopliae真菌对虫死亡率的综合影响.
主要方法:
- 在M. persicae上喷射针对CHS和TRE基因的dsRNA,然后用M. anisopliae进行治疗.
- 使用VIGS在植物中准mpTRE和mpCHS基因的dsRNAs的表达.
- 在M. persicae青虫中量化mpTRE和mpCHS基因的表达.
- 评估虫死亡率,生育率和存活率.
主要成果:
- 在与M. anisopliae结合时,射针对CHS和TRE基因的dsRNA显著增加了M. persicae的死亡率.
- 植物中针对mpTRE和mpCHS的dsRNAs的VIGS介导表达导致了虫中的基因表达减少.
- 暴露于VIGS表达的dsRNAs的虫表现出降低了生育率和存活率.
结论:
- 通过VIGS介导的RNA干扰是有效控制虫的有效方法.
- 将VIGS与M. anisopliae等虫病原菌结合起来可以提高害虫控制的有效性.
- 通过RNAi向TRE和CHS等必需基因,为昆虫害虫管理提供了一个可行的策略.
相关概念视频
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...