在不同料层上分化和未分化的精子干细胞的繁殖潜力
Kiana Sojoudi1,2, Hossein Azizi2, Maryam Solaimani2
1Faculty of Science, Department of Biology, University of Guilan, Rasht, Iran.
Cellular reprogramming
|March 20, 2025
概括
料层显著影响小鼠精子干细胞 (SSC) 培养. 不分化的SSC更喜欢MEF料,而分化的SSC在SNL和TSC料上生长最好,影响了精子生成研究.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞生物学 干细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 精子干细胞 (SSC) 对男性生育和遗传传输至关重要.
- 研究SSCs在体外是必不可少的,因为它们在体内很稀缺.
- 料层是必要的,以支持SSC在培养中的生存和繁殖.
研究的目的:
- 为了比较不同料层 (MEF,SNL,新生儿TSC,成人TSC) 对小鼠SSC培养的有效性.
- 用计算工具识别参与精子生成的关键基因和途径.
- 分析特定基因 (Dazl,Zbtb16,Stra8) 与料层和SSC差异化相关的表达模式.
主要方法:
- 在四个不同的料层上培养的小鼠SSC:MEF,SNL,新生儿TSC和成年TSC.
- 利用Cytoscape和STRING数据库进行蛋白质-蛋白质相互作用网络分析和基因丰富.
- 使用动态阵列芯片 (Fluidigm) 分析了Dazl,Zbtb16和Stra8的mRNA表达.
主要成果:
- 不分化的SSC在MEF料上显示出最佳的增长.
- 差异化的SSC在SNL和初级TSC料上显示出更好的长期培养生存.
- 发现的枢纽基因参与了细胞分化,半变和精子生成;基因表达因料类型和SSC分化阶段而异.
结论:
- 料层的选择对于未分化和分化小鼠SSC的成功体外培养至关重要.
- 已识别的枢纽基因和通路为精子生成的分子机制提供了洞察力.
- 不同的基因表达模式突出了料层对SSC行为和精子生成的影响.
更多相关视频
07:33A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
Published on: August 3, 2015
7.8K
09:38Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
Published on: June 21, 2012
90.7K
相关概念视频
Spermatogenesis
102.0K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.0K
Source And Potency Of Stem Cells
4.6K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
4.6K
Multipotency and Niche of Bulge Stem Cell
3.3K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.3K
Embryonic Stem Cells
3.4K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
3.4K
iPS Cell Differentiation
2.6K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.6K
