可光切换的超分子相互作用调节K+的跨膜运输和癌细胞亡
Cong Li1,2, Yaqi Wu1,2, Sheng Bao1
1College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou 311121, China.
Journal of the American Chemical Society
|March 20, 2025
概括
研究人员开发了一种仿生通道 (P3),模仿自然蛋白质. 这种光激活的通道切换离子运输状态,为了解蛋白质通道和治疗癌症等疾病提供了新的可能性.
科学领域:
- 生物仿真材料科学 生物仿真材料科学
- 超分子化学 超分子化学
- 分子生物物理学的分子生物物理学.
背景情况:
- 自然通道蛋白 (NCP) 呈现复杂的离子运输,难以人工复制.
- 人工离子运输系统缺乏NCPs复杂的监管机制.
研究的目的:
- 创建一个仿生通道 (P3),模仿NCP的多态离子运输.
- 为了研究对人工离子通道活动的光调节控制.
- 探索可切换的人工离子通道的治疗潜力.
主要方法:
- 合成P3.3形成的加入亚博的单链随机异质聚合物 (RHPs).
- 使用光诱导的阿佐基的光异构化和与β-环氧 (β-CD) 的宿主-客人相互作用.
- 研究P3在脂质体和癌细胞中的功能,监测离子流,ER压力,Ca2+信号,ROS产生和亡.
主要成果:
- P3 展示了光可控制的在三个离子运输状态 (ON,部分关闭,完全关闭) 之间切换.
- P3诱导了快速和大量的 (K+) 流出,导致ER压力,Ca2+火花,并增加了ROS.
- 人工通道触发了癌细胞中的线粒体依赖性亡.
结论:
- 基于亚博烯RHPs的P3通过光调节的超分子相互作用成功模拟了NCPs的多态传输.
- P3诱导癌细胞亡的能力突显了其治疗潜力.
- 这项工作为研究NCP机制和开发新疾病治疗提供了一个新的平台.
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