深度透和高分辨率的连续波非线性显微镜基于同类双发射上转换自适应光学
Jing Yao1,2,3, Zhipeng Yu1,3, Yufeng Gao2
1Department of Biomedical Engineering, Hong Kong Polytechnic University, Hong Kong SAR 999077, China.
Nano letters
|March 20, 2025
概括
用兰化物添加的升级转换纳米粒子 (UCNPs) 通过使用双排放来进行偏差校正和高分辨率可视化,使深层组织成像成为可能. 这一突破允许在复杂的环境中进行更清晰,更深入的生物成像.
科学领域:
- 生物医学光学 生物医学光学
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
背景情况:
- 添加兰他尼德的升级转换纳米粒子 (UCNPs) 在NIR激发下提供独特的双可见 (VIS) 和近红外 (NIR) 光子发射.
- 深层组织成像面临着与传统VIS和NIR发射的分辨率透权衡.
研究的目的:
- 开发一种连续波 (CW) 非线性显微镜技术,用于高分辨率,深层组织成像.
- 克服生物医学光学成像中的分辨率-透性限制.
主要方法:
- 使用的Tm3+/Yb3+联合剂的UCNP具有双重455nm (成像) 和800nm (偏差测量) 排放.
- 实施了一种同类的双排放上转换自适应光学系统.
- 采用了自制的非线性激光扫描显微镜,使用975nm CW激光.
主要成果:
- 在500μm深度的小鼠大脑组织中实现了接近衍射限制的成像 (侧面分辨率为480nm).
- 通过使用800nm辐射作为指南星,成功地纠正了深层组织中显著的光学偏差.
- 在具有挑战性的生物环境中,证明了同时进行高分辨率成像和偏差校正.
结论:
- 开发的CW非线性显微镜策略有效地扩展了UCNP在深层组织成像中的应用.
- 这种方法为探索深层组织光学特征提供了一种新的方法,具有增强的分辨率和透能力.
- 双排放UCNP系统为先进的生物医学成像挑战提供了一个有前途的解决方案.
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