有效的C25-维生素D的氧化3 使用人工自给自足的全细胞细胞染色体P450生物催化剂
Ziqi Liang1,2, Qiao Zhou1,2, Yicheng Li1,2
1College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Journal of agricultural and food chemistry
|March 20, 2025
概括
研究人员设计了自给自足的P450酶 (P450s) 来有效生物合成25-氧维生素D3. 计算分析优化了链接器,实现了这一关键维生素D代谢物的最高报告的全细胞产量.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 细胞染色体P450酶 (P450s) 显示了25-氧维生素D3 (25(OH) VD3) 生物合成的潜力.
- 工业应用P450s受到低稳定性,低效的催化和解反应的阻碍.
研究的目的:
- 开发自给自足的P450系统,用于增强25(OH) VD3的生产.
- 为了优化化学P450中的链接器动态,以提高催化性能.
主要方法:
- 整体细胞生物催化剂选和反应条件优化 (辅溶剂,细胞度,等离子体选择).
- 采用局部能量丧分析来评估蛋白质灵活性和全动力学.
- 构建并评估了带有优化链接器的模拟P450变体.
主要成果:
- 优化条件和heme域Vdh-K1实现了91.6%的转换效率.
- 鉴定出一种仿真P450变体VK1-CYP116B46-L21,具有增强的热稳定性,活性和合效率.
- 通过*Escherichia coli*全细胞催化实现了4.89mM (1.96g/L) 25(OH) VD3的创纪录产量.
结论:
- 计算式挫折分析对于完善多域酶中的链接器动态是有效的.
- 开发了一个可扩展和具有成本效益的框架,用于基于P450的高价值化合物的工业生物合成.
- 设计的P450系统显著提高了25(OH) VD3.3的生产.
相关概念视频
Role of Skin in Vitamin D Synthesis
4.9K
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
4.9K
Role of Vitamins in Maintaining Bone Health
3.1K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.1K
Synthesis and Functions of Calcitonin
1.5K
Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
1.5K
Cholesterol: Significance and Regulation
483
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
483


