高灵敏的双重质谱检测用于高分辨率的HILIC生物质燃烧标记物分离
Harrison Stevens1, Estrella Sanz Rodriguez2, Mingxia Lai1
1ARC Training Centre for Hyphenated Analytical Separation Technologies (HyTECH), Chemistry School of Natural Sciences, University of Tasmania, Hobart, Tasmania, Australia.
Journal of chromatography. A
|March 20, 2025
概括
一种新的方法可以准确地识别所有四种生物质燃烧的痕迹物,包括1,6-无-β-D-葡萄糖 (AGF),从而改善来源识别. 这项技术提高了环境样本分析的准确性,以检测莱夫葡萄 (LEV),曼诺 (MAN) 和银河 (GAL).
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 有机化学 有机化学
背景情况:
- 莱沃格卢科桑 (LEV),曼诺桑 (MAN) 和银河 (GAL) 是已确定的生物质燃烧痕迹剂.
- 另一种生物质燃烧同位素1,6-无-β-D-葡萄糖 (AGF) 很少被确定,这可能会影响以前的分析.
- AGF与其他异构体的共同化可能会损害生物质燃烧痕迹量化的准确性.
研究的目的:
- 开发一种用于从生物质燃烧中分离和量化四种关键单糖无水化物 (MAs) 的新方法.
- 为了能够准确地检测和定量1,6-无-β-D-葡萄糖 (AGF) 与已确定的标记物一起.
- 通过同位素比率分析,提高生物质燃烧源识别的可靠性.
主要方法:
- 使用HALO Penta-HILIC列的水友交互液体色谱 (HILIC) 方法的开发.
- 结合HILIC与双重三重四极质谱 (MS/MS) 进行敏感检测.
- 优化移动阶段,柱体温度,喷雾电压和选定的反应监测 (SRM) 过渡.
主要成果:
- 完成了LEV,MAN,GAL和AGF的基线分离和量化.
- 确定了检测的低极限:0.39微克/升 (LEV),0.62微克/升 (MAN),0.52微克/升 (GAL) 和0.04微克/升 (AGF).
- 使用NIST城市尘埃1649b验证的方法,首次报告了AGF度以及其他同位素的协议.
- 成功应用于各种环境矩阵,包括气溶,沉积物和冰芯.
结论:
- 开发的HILIC-MS/MS方法提供了一个快速 (<10分钟),选择性和敏感的方法来分析四个燃烧生物质的MA.
- 这种方法首次允许量化AGF,提高生物质燃烧痕迹研究的准确性.
- 使用所有四种标志物计算额外的异构体比率的能力可以提高生物质燃烧源的分配.
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