相关实验视频

Updated: May 11, 2026

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

新生山羊的β-葡萄糖训练免疫的免疫代谢效应

Miriam Angulo¹, Carlos Angulo¹

¹Immunology & Vaccinology Group and Laboratorio Nacional CONAHCYT (SECIHTI) de Generación de Vacunas Veterinarias y Servicios de Diagnóstico, Centro de Investigaciones Biológicas del Noroeste, S.C., Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz, B.C. S. C.P. 23096, Mexico.

Research in veterinary science

|March 20, 2025

概括

德巴里奥梅西斯汉森尼 (Debaryomyces hansenii) 的β-葡萄糖通过促进糖解来增强新生山羊的训练免疫力. 这些对免疫记忆至关重要的代谢转变在单细胞中观察到,并且不受NLRP3抑制的影响.

科学领域:

免疫学免疫学免疫学
代谢学代谢学代谢学
菌类学菌类学是指菌类学.

背景情况:

众所周知,Debaryomyces hansenii β-葡萄糖可以诱导训练免疫力.
基本的代谢转变和信号通路在很大程度上仍未被描述.

研究的目的:

在培训免疫诱导和抑制过程中研究代谢变化. in vitro.
在体内探索与β-葡萄糖诱导的免疫记忆相关的代谢变化和信号通路.

主要方法:

在体外脂聚糖 (LPS) 再刺激模型与β-葡萄糖和MCC950.0.
来自经过β-葡萄糖治疗的山羊的单细胞的ex vivo再刺激与细菌病原体.
对代谢概况和基因表达的分析 (AKT/MTOR/HIF1Α,Dectin1,TLR4,TLR6,RAF1,IL1Β,IL6,TNFΑ).

主要成果:

免疫训练在体外增强了糖解代谢物 (葡萄糖和乳酸),独立于NLRP3抑制.
在体内口服β-葡萄糖的使用会增加单细胞中的糖分解代谢产物.
在单细胞中观察到的AKT/MTOR/HIF1Α信号通路和特定免疫基因 (Dectin1,TLR4,TLR6,RAF1,IL1Β,IL6) 的升调.
在单细胞中降低TNFα基因表达的调节.

结论:

德巴里奥梅西斯汉森尼 (Debaryomyces hansenii) 的β-葡萄糖通过糖解转移诱导新生山羊单细胞的训练免疫力.
这些代谢变化没有被MCC950逆转,这表明NLRP3独立的机制.
这项研究阐明了参与β-葡萄糖介导训练免疫的关键代谢和遗传途径.

关键词:

动物健康动物健康功能性碳水化合物是一种功能性碳水化合物.在先天白细胞中的记忆.代谢转移是指代谢的转移.

更多相关视频

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Published on: July 31, 2021

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

相关概念视频

Immunological Memory

Immunological Memory

Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...

Development of Immunocompetence

Development of Immunocompetence

The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...