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CtMYB63通过JA信号通路增强了松花的浸水耐受性

Yingqi Hong¹, Shiwen Zhou², Jianyi Zhang³

¹College of Horticulture, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, 130118, China; Institute for Safflower Industry Research / Pharmacy School of Shihezi University, Shihezi, 832003, China.

Plant physiology and biochemistry : PPB

|March 20, 2025

概括

甲基斯酸盐 (MeJA) 增强了红杉的功效.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

浸水压力是对松花产量的主要威胁.
甲基斯酸盐 (MeJA) 在红杉对浸水的反应中的作用尚未完全理解.

研究的目的:

阐明MeJA增强红杉对水浸压力的耐受性的机制.
为了研究CtMYB63基因在这个过程中的功能.

主要方法:

对基因表达的分析 (CtMYB63,CtDFR1,CtANS1,CtANR1) 进行.
过度表达和基因删除研究 (CtMYB63,CtMYB63Δ).
对抗氧化酶活性和活性氧物种的生物化学测定.
酵母单杂交 (Y1H) 和光酶测定用于基因调节研究.

主要成果:

通过TGACG动机,MeJA诱导了CtMYB63的表达.
CtMYB63调节下游基因 (CtDFR1,CtANS1,CtANR1),增加生物质和黄类,同时抑制延长.
过度表达CtMYB63增强了抗氧化活性,并改善了对水浸压力的耐受性.
MeJA进一步提高了野生类型和CtMYB63过度表达的红杉的浸水耐受性.
在CtMYB63Δ中删除TGACG基因减弱了浸水耐受性.
CtMYB63与基因促进体结合,而CtJAZ9则起到抑制作用.

结论:

CtMYB63在通过斯酸盐 (JA) 信号通路增强松花的浸水耐受性方面发挥着至关重要的作用.
这项研究提供了一种新的分子育种策略,用于在水浸条件下提高树叶的产量.

关键词:

在CtMYB63中使用.梅杰亚·梅杰亚是什么意思沙夫花花的花朵是什么意思水浸压力是水浸压力的压力.

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