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17β-雌激醇和雌激素受体亚型特异性激动剂对Jurkat E6.1 T细胞白血病细胞的影响

Rahul S Nair¹, Mantavya N Patel², Thangamani Kannan²

¹Inspire Laboratory, Institute of Advanced Research in Health Sciences, Tamil Nadu Government Multi Super Speciality Hospital, Chennai, Tamil Nadu, India; Integrative Medicine Laboratory, Department of Biotechnology, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

Toxicology in vitro : an international journal published in association with BIBRA

|March 20, 2025

概括

雌激素信号影响T细胞白血病,通过将细胞代谢从糖解转移到氧化酸化. 这涉及关键的酶和信号通路,影响Jurkat E6.1白血病细胞的细胞存活率.

科学领域:

细胞代谢的细胞代谢.
免疫学免疫学免疫学
在瘤学瘤学.

背景情况:

雌激素信号传递对于免疫调节和癌症代谢至关重要.
它在T细胞白血病中的作用,特别是影响生存和增殖的代谢转变,尚未完全理解.
雌激素受体 (ER) 的激活可能会影响血液恶性瘤中的这些代谢变化.

研究的目的:

研究17β-雌激醇和ER亚型特异性激动剂对Jurkat E6.1 T细胞白血病细胞的体外作用.
评估雌激素信号对代谢重编程,炎症反应和生存途径的影响.
阐明所涉及的受体依赖和独立机制.

主要方法:

尤卡特E6.1细胞被用17β-雌激醇,ER-α或ER-β激动剂在不同度下治疗.
测量了关键的代谢酶活动 (酸酶,酸酶,酸合成酶).
评估了介质素-6 (IL-6),氧化 (NO) 生产和PI3K/Akt通路激活 (p-Akt/总Akt).

主要成果:

17β-雌激醇治疗诱导了从糖解到氧化酸化的代谢转变.
观察到赫索基纳酶活性显著下降.
同时注意到酸盐激酶和酸盐合成酶活动的增加.

结论:

17β-雌二醇通过受体亚型依赖和独立的途径影响Jurkat E6.1细胞.
这些效应包括对代谢酶,细胞因子 (IL-6),氧化和信号分子 (p-Akt) 的调节.
雌激素信号传递在T细胞白血病细胞的代谢重编程中发挥作用.

关键词:

17β-雌二醇是17β-雌二醇中的一种.这就是ER-α.这是ER-ββ.在Jurkat E6.1细胞中.代谢重编程是一种代谢重编程.

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