在饥饿压力下的Apostichopus japonicus肠道降解的非编码RNA调节网络:从转录组分析的见解
Lifei Ge1, Ying Wei1, Zhiqing Ye1
1National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, People's Republic of China.
International journal of biological macromolecules
|March 20, 2025
概括
饥饿压力影响海黄,长非编码RNAs (lncRNAs) 调节肠道再生. 确定了关键的基因轴,为开发抗压水产养殖品种提供了洞察力.
科学领域:
- 海洋生物学 海洋生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 饥饿是水生动物常见的压力因素,诱导补偿生长.
- 海 (Apostichopus japonicus) 使用长非编码RNA (lncRNAs) 适应环境变化并再生组织.
- 了解IncRNA在饥饿反应中的作用对于水产养殖至关重要.
研究的目的:
- 研究lncRNAs在饥饿压力下的海黄瓜肠道降解和再生中的作用.
- 确定参与补偿生长的关键lncRNA-mRNA相互作用.
- 阐明管理A. japonicus.饥饿反应的调节网络.
主要方法:
- 从饥饿的海黄瓜肠道中获得的转录基因数据的高通量链特异性测序.
- 识别和分类高质量的 lncRNAs.
- 对差异表达的mRNA和lncRNA进行查.
- 竞争性内源RNA (ceRNA) 网络模型的构建.
主要成果:
- 确定了与肠道降解相关的关键 lncRNA 和 mRNA.
- 发现了重要的调节轴 (例如,AJSOX9/Aja-miR-2012-5p/MSTRG.2956.1,AJWNT9B/Aja-miR-200-3p/MSTRG.19757.1) 参与肠道再生.
- 发现了AjFABP2/Aja-miR-9-5p/MSTRG.9667.1轴调节饥饿期间的能量代谢.
结论:
- 在饥饿的海中,lncRNAs在调节肠道再生和能量代谢方面发挥着至关重要的作用.
- 已识别的ceRNA网络提供了关于无脊椎动物对饥饿压力的反应的见解.
- 这些发现支持开发耐压力海菌株,以实现可持续的水产养殖.
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