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相关概念视频

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.3K
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

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The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.0K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
862
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

7.0K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
7.0K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

6.3K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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相关实验视频

Updated: May 21, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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通过差异变量基因分析探索细胞间的变异性和功能洞察力.

Victoria Gatlin1,2, Shreyan Gupta1,2, Selim Romero1,2,3

  • 1Department of Veterinary Integrative Biosciences, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.

NPJ systems biology and applications
|March 21, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了spline-DV,一种用于分析单细胞RNA测序数据中的基因表达变异性的新方法. 它揭示了表达变异性改变的基因,为细胞功能提供了比平均表达水平更深入的见解.

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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
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相关实验视频

Last Updated: May 21, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

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Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
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科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 系统生物学 系统生物学

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 提供了高分辨率的基因表达数据.
  • 细胞间的变异性对于细胞功能至关重要,但经常被忽视.
  • 当前差异表达式 (DE) 分析侧重于平均表达式,忽略了变化.

研究的目的:

  • 在scRNA-seq数据中开发差异变性 (DV) 分析的统计框架.
  • 为了识别具有显著变化的表达变异性在条件之间的基因.
  • 通过分析基因表达变异性,更深入地了解细胞系统.

主要方法:

  • 引入了spline-DV,一个新的统计框架.
  • 在scRNA-seq数据集中应用spline-DV.
  • 鉴定差异变量的基因.

主要成果:

  • Spline-DV有效地识别出表达变异性发生显著变化的基因.
  • 已识别的DV基因在功能上与细胞状况相关.
  • 案例研究表明在肥胖,纤维化和癌症研究中有用.

结论:

  • 差异变性分析为差异表达式分析提供了补充的见解.
  • Spline-DV增强了对细胞异质性和功能的理解.
  • 这种方法推进了用于生物发现的scRNA-seq数据的分析.