相关实验视频

Updated: Jul 8, 2026

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

BMAL1通过激活SIRT1/PGC-1α通路来减轻椎间盘退化:来自体外研究的证据

Peiming Sang^1,2, Yanyan Ma^3,4, Xie Zhang^1,2

¹Ningbo Medical Center LiHuiLi Hospital, Ningbo, Zhejiang, People's Republic of China.

Scientific reports

|March 21, 2025

概括

大脑和肌肉arnt-like蛋白-1 (BMAL1) 可能通过激活SIRT1/PGC-1α通路来抑制椎间盘退化 (IVDD). 这项研究探讨了BMAL1.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

椎间盘退化 (IVDD) 是一个复杂的病理过程.
生物节律蛋白,如BMAL1,在IVDD中的作用尚未完全理解.
确定IVDD的新型治疗点至关重要.

研究的目的:

在实验室中研究大脑和肌肉ARNT样蛋白-1 (BMAL1) 对椎间盘退化 (IVDD) 的影响.
阐明潜在的分子机制,特别是SIRT1/PGC-1α通路的参与.

主要方法:

在体外研究中利用了不朽化的核肉质细胞 (NP).
使用siRNA和pcDNA载体操纵BMAL1表达.
评估了NP细胞特征,包括活性,密度,细胞亡,炎症,ROS,衰老和线粒.
评估了BMAL1,SIRT1和PINK1.1的表达.

主要成果:

过度表达BMAL1增加了SIRT1和PGC-1α的表达.
过度表达BMAL1降低了炎症反应,ROS水平,亡和衰老.
过度表达BMAL1增强了NP细胞的活性,密度和线粒细胞衰变.
在体外,BMAL1有效抑制了IVDD的进展.

结论:

在体外,BMAL1对IVDD进展起着保护作用.
BMAL1通过激活SIRT1/PGC-1α通路来发挥其作用.
BMAL1代表了管理IVDD的潜在治疗标.

关键词:

在 BMAL1 中,椎间盘退化症 (IVDD) 是一种疾病.线粒细胞衰变 - - 线粒细胞衰变 (mitophagy) 是一种有关SIRT1/PGC-1α 的SIRT1/PGC-1α 的SIRT1.

更多相关视频

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits

Published on: July 21, 2023

相关概念视频

Herniated Intervertebral Disc l: Introduction

Herniated Intervertebral Disc l: Introduction

Intervertebral disc herniation refers to the displacement of the nucleus pulposus (the gel-like inner core of the disc) through a tear or weakened area in the annulus fibrosus (the outer fibrous ring). The displaced disc material extends beyond the normal boundaries of the disc space and may compress or irritate nearby spinal nerve roots or, less commonly, the spinal cord.Etiology and Risk FactorsHerniation commonly results from degeneration, in which aging reduces disc hydration and...

Degenerative Disc Disease I: Introduction

Degenerative Disc Disease I: Introduction

Degenerative disc disease is a chronic condition in which intervertebral discs gradually lose structure and function. It is not infectious or autoimmune; rather, it results from age-related biochemical and mechanical changes, influenced by genetic, metabolic, and environmental factors.Structure and Function of DiscsThe spine contains 23 intervertebral discs that absorb load, distribute forces, maintain spacing, and allow flexibility. Each disc consists of a nucleus pulposus, a gel-like core...

Degenerative Disc Disease ll: Pathophysiology

Degenerative Disc Disease ll: Pathophysiology

The symptoms of degenerative disc disease arise from a combination of mechanical compression, vascular compromise, and biochemical inflammation, which together disrupt nerve function and produce pain.Mechanical CompressionDisc degeneration reduces height and elasticity, predisposing to herniation of the nucleus pulposus, a major cause of radicular pain. Herniations may be protrusion (bulging with intact annulus), extrusion (nucleus extends beyond disc but remains connected), or sequestration...