一种基于银纳米颗粒的绿色合成的创新方法,使用石榴皮提取物用于抗菌目的
Rocío Díaz-Puertas1, Francisco J Álvarez-Martínez1, Enrique Rodríguez-Cañas1
1Institute of Research, Development and Innovation in Healthcare Biotechnology in Elche (IDiBE), Miguel Hernández University, Elche 03202, Alicante, Spain.
Bioinorganic chemistry and applications
|March 21, 2025
概括
使用石榴皮提取物进行银纳米粒子 (AgNPs) 的绿色合成,通过Python编程的Box-Behnken设计进行了优化. 由此产生的AgNP显示出显著的抗菌活性,显示出生物医学应用的潜力.
科学领域:
- 绿色化学 绿色化学
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
背景情况:
- 由于环境和经济原因,纳米材料的可持续合成至关重要.
- 石榴皮提取物含有生物活性化合物,如punicalagin,适合纳米粒子合成.
- 优化合成参数对于可复制和高效的纳米材料生产至关重要.
研究的目的:
- 开发一种使用石榴皮提取物为银纳米粒子 (AgNPs) 的绿色合成方法.
- 使用Python编程的Box-Behnken设计 (BBD) 来优化合成过程.
- 描述合成的AgNP并评估它们的抗菌活性和潜在的生物医学应用.
主要方法:
- 绿色合成AgNP使用石榴皮提取物.
- 使用Box-Behnken设计优化合成参数 (酸银度,提取物度,温度).
- 使用UV-Vis,FTIR,XRD和FESEM进行AgNP的表征.
- 对抗大肠杆菌和金黄色葡萄球菌的抗菌活性的评估.
- 在纳米纤维支架中纳入AgNP.
主要成果:
- 优化的条件产生了具有理想特征的AgNP (水力动力直径,PDI,泽塔潜力).
- 鉴定证实了AgNPs的形成.
- 合成的AgNP对大肠杆菌和黄金色杆菌具有显著的抗菌活性.
- 当纳米纤维支架中纳入时,AgNPs保留了部分抗菌活性.
结论:
- 石榴皮提取物是绿色AgNP合成的可持续和有效媒介.
- 盒子-Behnken设计是优化纳米材料合成过程的宝贵工具.
- 合成的AgNP显示出在生物医学领域的抗菌应用的前景.
更多相关视频
11:19Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
Published on: May 10, 2018
10.2K
11:52Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
2.9K
相关概念视频
01:13Special Staining Techniques
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
01:28Biological Methods for Microbial Control
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
01:24Microbial Corrosion
Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
01:27Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
01:24iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
01:25Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...