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检测尿病原性大肠杆菌的病毒性决定因素

Nivetha R M¹, Shanthi Mariappan¹, Uma Sekar¹

¹Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.

|March 21, 2025

概括

这项研究研究了尿病原性大肠杆菌 (UPEC) 的毒性因素和抗生素耐药性. 最常见的是 fimH 基因,对安皮西林和西普罗夫洛克萨的耐药性较高.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
遗传学是一个遗传学.

背景情况:

大肠杆菌是尿路感染 (UTI) 的主要原因.
耐多药性大肠杆菌的增加需要了解毒性因素和耐药性模式.
识别毒性决定因素有助于理解尿路感染病原和治疗.

研究的目的:

在尿病原性大肠杆菌 (UPEC) 隔离物中对毒性因子进行遗传特征化.
为了确定这些UPEC菌株的抗生素敏感性概况.
探索特定毒性基因与抗菌素耐药性之间的潜在相关性.

主要方法:

分析了150个连续的大肠杆菌尿液分离物.
聚合酶链反应 (PCR) 用于检测毒性基因:fimH,iutA,hlyA,papC,cnf1和sat.
使用磁盘扩散方法对十种抗生素进行了抗生素敏感性测试.

主要成果:

在150个分离物中,112个分离物中至少含有一个毒性基因.
最常见的基因是fimH (77%),iutA (57%) 和sat (45%);没有papC.
对安皮西林 (88%) 和西普洛素 (85%) 观察到高耐药率.
68%的分离物是扩展光谱β-乳糖酶 (ESBL) 生产者,12%是耐卡巴胺的大肠杆菌 (CREC).

结论:

fimH是UPEC中最常见的毒性因子.
在特定的毒性基因和抗菌素耐药性概况之间没有发现显著的关联.
产生ESBL和CREC的高患病率凸显了治疗大肠杆菌引起的尿路感染的挑战.

关键词:

抗微生物耐药性抗微生物耐药性细胞毒性消亡因子1 美国大肠杆菌 (E. coli)在p fimbriaee中使用.欧佩克 (Upec) 是一个国际组织.尿路感染尿路感染毒性因素是毒性的因素.α-血溶素的使用情况

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