3D生物打印多层细胞结构,具有渐变核心外接口,用于肌至骨组织再生
WonJin Kim1, Dong Rak Kwon2, Hyeongjin Lee3
1Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea.
Bioactive materials
|March 21, 2025
概括
这项研究引入了一种用于旋转手套撕裂的新型生物打印方法,创建工程肌到骨组织. 这种方法成功地再生了复杂的组织,改善了动物模型中的愈合和整合.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 旋转手套撕裂在活跃个体中很普遍,由于自然愈合不佳,经常需要手术.
- 目前的治疗方法在完全恢复本地肌至骨复杂性和功能方面存在局限性.
研究的目的:
- 开发一种生物打印策略,用于制造工程肌至骨复杂组织.
- 创建一个渐变接口,模仿本地肌骨结,以增强整合.
- 评估生物打印构造物用于旋转手腕再生的体外和体内疗效.
主要方法:
- 使用了脱细胞化细胞外基质生物墨水与酸和TGF-β/多乙烯醇).
- 采用核心外喷嘴生物打印系统,同时制造对齐的肌,梯度接口和骨区域.
- 使用人体脂肪干细胞in vitro和子旋转手套撕裂模型in vivo评估构造性能.
主要成果:
- 实现了干细胞向骨质生和肌质生血统的有针对性的分化.
- 通过分级接口结构证明了增强的纤维软骨形成和肌骨融合in vitro.
- 观察到全厚肌至骨组织的显著再生,包括高质量的接口,改善的机械特性,血管生成和ECM形成in vivo.
结论:
- 拟议的生物打印方法有效地再生复杂的肌至骨组织.
- 梯度接口设计对于优异的组织整合和愈合至关重要.
- 这个平台显示出治疗旋转手腕损伤和其他肌肉骨缺陷的巨大潜力.
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