相关实验视频

Updated: May 21, 2025

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

以数据为导向的评估适合的免疫原来改善抗体选择

Katharina Waury^1,2, Hlin Kvartsberg^3,4, Henrik Zetterberg^3,4,5,6,7,8

¹Department of Computer Science, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.

Protein science : a publication of the Protein Society

|March 21, 2025

概括

选择正确的蛋白质片段,或免疫原体,用于抗体生产是关键. 较短的免疫原体具有无序的区域和线圈延伸产生成功的抗体,而β片和二硫化物桥梁阻碍了性能.

科学领域:

免疫学和生物技术
生物信息学和计算生物学

背景情况:

抗体是研究和临床环境中的关键试剂,因为它们具有特定的抗原结合能力.
为产生抗体选择最佳的蛋白质片段 (免疫原体) 是一个重大挑战,影响研究可重现性.

研究的目的:

系统地评估影响抗体生产成功和特异性的免疫原特性.
确定与抗体性能相关的免疫原的关键结构和功能特征.
开发一种工具,以帮助研究人员选择适合抗体开发的免疫原.

主要方法:

利用人类蛋白质图谱来分析免疫原特性.
根据标准化验证实验将抗体分类为成功或失败的.
进行免疫原序列的结构和功能分析,包括长度,二次结构和翻译后修改位点.

主要成果:

较短的免疫原体 (≤50残留物),具有无序的N/C末端和长线圈区域,与更高的成功率有关.
富含β薄膜,跨膜区域或二硫化物桥梁的免疫原与较差的抗体性能相关.
免疫原体内的翻译后修饰部位表明了对抗体生成具有潜在益处的区域.

结论:

免疫原特征显著影响抗体的成功和特异性.
开发了R包"immunogenViewer",以帮助研究人员选择最佳的免疫原体.
改善免疫原选择可以提高科学研究中的抗体可靠性和可重现性.

关键词:

人类蛋白质图书馆人类蛋白质图书馆抗体是一种抗体.抗原是一种抗原.这是一种混乱的混乱,一种混乱的混乱.免疫原的免疫原的作用.蛋白质结构蛋白质结构

更多相关视频

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Antigenic Liposomes for Generation of Disease-specific Antibodies

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

相关概念视频

Immunological Memory

Immunological Memory

Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...