多层自催化反能够在资源竞争和跨度范围内实现综合控制
Armin M Zand1, Stanislav Anastassov1, Timothy Frei1
1ETH Zurich, Department of Biosystems Science and Engineering, Schanzenstrasse 44, Basel 4056, Switzerland.
ACS synthetic biology
|March 21, 2025
概括
多层反控制器可以在合成生物学中实现强大的蛋白质表达调节. 这些新的设计实现了人口层面和多细胞控制,克服了对增强细胞功能的资源竞争.
科学领域:
- 合成生物学 合成生物学
- 控制理论 控制理论 控制理论
- 微生物生态学 微生物生态学
背景情况:
- 综合反控制对于在动态细胞环境中强大的蛋白质表达至关重要.
- 自动催化一体反控制器提供了简单性和稳定性,但面临着资源竞争等局限性.
- 现有策略需要进步,以充分利用复杂生物系统中的控制器潜力.
研究的目的:
- 通过引入多层反策略来解决当前集成反控制器的局限性.
- 为合成生物学应用开发种群级和多细胞一体反控制器.
- 为在遗传网络中模拟资源竞争提供一个数学框架.
主要方法:
- 为自催化控制器设计和实施多层反策略.
- 开发了一个通用的数学框架来模拟遗传网络中的资源竞争.
- 在合成生物学中的度调节和比度控制任务中应用控制器.
主要成果:
- 通过协调细胞群体相互作用,实现了人群级整体反和多细胞集成器.
- 证明了基因表达,基因比率,人口增长和共同培养成分的强有力的调节.
- 在工程微生物生态系统中验证了控制器的有效性,展示了跨生物尺度的适应性.
结论:
- 多层自催化控制器为强大的适应和恒温提供了一种多功能方法.
- 开发的框架有助于设计细胞内控制电路和多细胞系统.
- 这项工作通过从亚细胞到多细胞层次的精确控制来推进合成生物学.
相关概念视频
Effects of feedback
496
Feedback in control systems plays a critical role in shaping various operational parameters, extending beyond simple error reduction to influence stability, bandwidth, gain, impedance, and sensitivity. Understanding these effects requires examining a basic feedback system characterized by defined input, output, error, and feedback signals.
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
496
Feedback control systems
262
Feedback control systems are categorized in various ways based on their design, analysis, and signal types.
Linear feedback systems are theoretical models that simplify analysis and design. These systems operate under the principle that their output is directly proportional to their input within certain ranges. For instance, an amplifier in a control system behaves linearly as long as the input signal remains within a specific range. However, most physical systems exhibit inherent nonlinearity...
Linear feedback systems are theoretical models that simplify analysis and design. These systems operate under the principle that their output is directly proportional to their input within certain ranges. For instance, an amplifier in a control system behaves linearly as long as the input signal remains within a specific range. However, most physical systems exhibit inherent nonlinearity...
262
Control Systems
987
Control systems are everywhere in contemporary society, influencing diverse applications from aerospace to automated manufacturing. These systems can be found naturally within biological processes, such as blood sugar regulation and heart rate adjustment in response to stress, as well as in man-made systems like elevators and automated vehicles. A control system is essentially a network of subsystems and processes that collaboratively convert specific inputs into desired outputs.
At the heart...
At the heart...
987
Open and closed-loop control systems
594
Control systems are foundational elements in automation and engineering. They are broadly categorized into open-loop and closed-loop systems. These classifications hinge on the presence or absence of feedback mechanisms, significantly influencing the system's performance, complexity, and application.
An open-loop control system operates without feedback from the output. It consists of two primary elements: the controller and the controlled process. The controller receives an input signal...
An open-loop control system operates without feedback from the output. It consists of two primary elements: the controller and the controlled process. The controller receives an input signal...
594
Cell Signaling Feedback Loops
6.2K
Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
6.2K
Load-frequency control
106
Load-frequency control (LFC) is vital for maintaining power system stability, ensuring that frequency and power flows remain within acceptable limits during load changes. Turbine-governor control eliminates rotor accelerations and decelerations following load changes. However, a steady-state frequency error persists when the change in the turbine-governor reference setting is zero. In an interconnected power system, each area agrees to export or import a scheduled amount of power through...
106


