在对潜在的传染性样品进行分类之前,检查气溶疏散系统的正常功能
Kristen M Reifel1, Avrill Aspland2, Suat Dervish3
1Flow Cytometry Core, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Current protocols
|March 21, 2025
概括
一种新方法验证了使用光珠的细胞分类器气溶疏散系统,以确保操作员的安全. 这确保了仪器故障时的危险气溶的制,保护实验室人员.
科学领域:
- 生物技术是生物技术.
- 实验室的安全工作.
- 气溶科学 气溶科学
背景情况:
- 细胞分类器在仪器故障时可以产生危险的气溶,对操作员构成风险.
- 目前的气溶控制依赖于疏散系统和生物安全柜,但疏散系统需要定期核实.
- 在细胞分类中处理传染性或危险样本时,操作人员的安全至关重要.
研究的目的:
- 描述一项更新的协议,用于验证从细胞分类器中的气溶的制和疏散.
- 确保气溶疏散系统在处理危险样品之前正常运行并提供足够的保护.
- 验证工程控制在防止操作员暴露于气溶中的有效性.
主要方法:
- 模拟仪器故障通过破坏细胞分类器流并产生气溶.
- 使用1μm光珠制造可检测的气溶颗粒.
- 在分类采集区域外采集空气样本,使用冲击器式气溶样本.
- 检查收集的样本是否存在光珠,以检测气溶泄漏.
主要成果:
- 空气样本中没有珠子表明有足够的气溶封闭和疏散.
- 珠子的存在意味着气溶疏散系统或其他控制系统的潜在故障.
- 该协议为气溶疏散系统提供了用户驱动的验证方法.
结论:
- 定期验证气溶疏散系统对于操作员安全至关重要.
- 描述的协议提供了一种可靠的方法来检测气溶封装故障.
- 确保气溶疏散系统的正确功能对于使用细胞分类器时的安全实验室实践至关重要.
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