Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cofactors and Coenzymes01:27

Cofactors and Coenzymes

80.7K
Enzymes require additional components for proper function. There are two such classes of molecules: cofactors and coenzymes. Cofactors are metallic ions and coenzymes are non-protein organic molecules. Both of these types of helper molecule can be tightly bound to the enzyme or bound only when the substrate binds.
80.7K
Amines to Sulfonamides: The Hinsberg Test01:23

Amines to Sulfonamides: The Hinsberg Test

3.2K
The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...
3.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hypophosphite Is a Naturally Occurring Selective Inhibitor of Syntrophic Methanogenesis.

Environmental science & technology·2026
Same author

Polyhydroxyalkanoates as ecological currencies across the microbial tree of life.

Advances in microbial physiology·2026
Same author

Phage-based microbiome manipulation reveals ecological interactions within gut communities.

bioRxiv : the preprint server for biology·2026
Same author

Organic carbon oxidation state shapes fermentative methanogenic microbiomes and controls greenhouse gas fluxes.

Nature communications·2026
Same author

Extensive spontaneous genome reduction in Paraburkholderia sabiae.

Scientific reports·2026
Same author

High-throughput characterization of Mycobacterium tuberculosis gene function across diverse conditions.

PLoS biology·2026

相关实验视频

Updated: May 21, 2025

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

9.6K

微生物在胺胺偏好方面表现出广泛的多样性.

Kenny C Mok1, Olga M Sokolovskaya1, Adam M Deutschbauer1,2

  • 1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California, USA.

mSystems
|March 21, 2025
PubMed
概括

微生物对维生素B12 (cobamide) 形式的偏好不同,这影响了它们的生长和社区角色. 这项研究使用纯化,罕见的化物来表征细菌的化物偏好.

关键词:
科巴拉胺是什么意思科巴化物是一种科巴胺的偏好情况这是一种腐蚀性腐蚀物.维生素B12 维生素B12 是一种

更多相关视频

Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

13.5K
Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

448

相关实验视频

Last Updated: May 21, 2025

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

9.6K
Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

13.5K
Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

448

科学领域:

  • 微生物学 微生物学
  • 生物化学 生物化学
  • 营养科学 营养科学

背景情况:

  • 科巴胺 (维生素B12家族) 是许多生物体必需的辅助因子,但由少数核细胞产生的.
  • 胺体现出显著的结构多样性,在微生物群落中发现了各种形式.
  • 现有的科巴胺偏好研究受限于大多数科巴胺形式的商业不可用性.

研究的目的:

  • 描述细菌对各种商业上不可用的胺基的偏好.
  • 研究不同形式的胺如何影响细菌生长和代谢过程.
  • 了解在微生物群落中胺多样性的生态影响.

主要方法:

  • 提取和净化七种商业上无法获得的胺.
  • 使用工程 *Escherichia coli*, *Sinorhizobium meliloti* 和 *Bacillus subtilis* 菌株进行生长实验.
  • 功能性隔离特定的胺依赖途径,如甲氨酸合成.

主要成果:

  • 在不同细菌物种之间甚至在同一生物体内的代谢途径之间观察到胺偏好的显著差异.
  • 微生物的不同之处在于它们最有效地利用的胺,不支持生长的胺,以及所需的最小度.
  • 在不同生物体中,生长效率高达四个数量级,在生物体内的酶之间高达20倍.

结论:

  • 细菌对胺的偏好非常多样化,影响生长和代谢功能.
  • 这些偏好可能在塑造微生物社区结构和功能方面发挥着关键作用.
  • 对胺生物学的进一步研究对于理解微生物生态学和营养循环至关重要.