相关实验视频

Updated: May 21, 2025

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

通过针对防御岛屿,有效地对Shewanella进行基因操纵

Yilong Ruan^1,2,3, Huan Tang^1,2,3, Tongxuan Cai^1,4

¹Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

Applied and environmental microbiology

|March 21, 2025

概括

我们在Shewanella putrefaciens CN32中识别并删除了防御岛屿,显著提高了基因修饰的效率. 这一策略增强了Shewanella的作用.

科学领域:

微生物学微生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

舍瓦尼拉菌种具有对生物修复和微生物燃料电池至关重要的呼吸适应性.
细菌的防御系统,包括限制修饰和CRISPR,阻碍了基因操纵.
防御岛屿,是移动元件上的防御系统集群,对基因工程构成重大障碍.

研究的目的:

开发一种系统的方法来预测,绘制和分析细菌基因组中的防御岛屿.
调查和删除遗传反抗的Shewanella putrefaciens CN32中的防御岛屿,以加强遗传修饰.
评估这些防御岛屿在其他Shewanella菌株和グラム阴性细菌中的流行程度.

主要方法:

整个基因组的预测和防御岛屿的绘制.
在保存的染色体基因 (trmA和trmE) 中集成的防御系统的识别.
使用过度表达切除酶的防御岛屿切除的实验验证.
在防御岛屿移除之前和之后评估基因操纵效率.

主要成果:

在Shewanella putrefaciens CN32中确定了两个关键的防御岛屿,位于trmA和trmE基因内.
切除酶的过度表达成功地促进了这些防御岛屿从染色体中去除.
删除防御岛屿显著提高了S. putrefaciens CN32.32的基因操纵效率.
发现防御岛屿在各种Shewanella菌株和其他克拉姆阴性细菌中广泛存在.

结论:

针对性地移除防御岛屿是克服Shewanella遗传障碍的有效策略.
这种方法显著提高了基因操纵的效率,释放了Shewanella在环境和微生物工程方面的潜力.
这些发现表明,防御岛屿向修改对于优化生物技术中的微生物过程具有更广泛的适用性.

关键词:

谢瓦内拉 (Shewanella) 是一个美丽的女孩.谢瓦内拉 (Shewanella) 是一个腐烂的生物.防御系统的防御系统.遗传操纵遗传操纵是一种基因组岛屿的基因组岛屿

更多相关视频

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

相关概念视频

What is Genetic Engineering?

What is Genetic Engineering?

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Transgenic Organisms

Transgenic Organisms

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...