小型线粒体基因组的描述和表征Trichoderma cerinum (Hypocreales,Hypocreaceae) 和它的进化观点
Priscila Martins de Assis1, Igor Henrique Rodrigues-Oliveira1,2, Iuri Batista da Silva1,2,3
1Laboratory of Ecological and Evolutionary Genetics, Federal University of Viçosa, Rio Paranaíba, Brazil.
概括
在Trichoderma cerinum的线粒体基因组组装和注释. 遗传学分析显示,T. cerinum是包括T. lixii和T. harzianum在内的一个分类的姐妹群.
科学领域:
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 特里科德尔马 (Trichoderma) 是一种在全球分布的真菌属,在土壤和腐烂的木材中发现.
- 了解真菌线粒体基因组对于进化研究至关重要.
研究的目的:
- 为了组装和注释Trichoderma cerinum的线粒体基因组.
- 评估Trichoderma物种的系系,并了解真菌进化中的基因增益/损失.
主要方法:
- 对T. cerinum的原始测序数据是从NCBI获得的.
- 在Galaxy Europe平台上使用NOVOplasty进行了线粒基因组组装.
- 遗传学分析包括12个Trichoderma物种和Fusarium oxysporum作为一个外群.
主要成果:
- 对T. cerinum. 获得了26,696bp的循环化线粒体基因组.
- 该基因组包含15个蛋白质编码基因,25个tRNA,两个rRNA,两个内核酶序列和一个orf40.
- 遗传学重建将T. cerinum作为一个由T. lixii,T. afroharzianum,T. simmonsii和T. harzianum组成的分类的姐妹群.
结论:
- 这项研究提供了Trichoderma cerinum的第一个完整的线粒体基因组序列.
- 这些发现有助于理解Trichoderma属内的进化历史和遗传学关系.
- 对基因含量和家族遗传位置的分析为真菌基因组进化提供了洞察力.
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