利用细菌外聚糖生物转化利用剩余奶酪乳糖作为培养基
Elaine Cristina da Silva1, Leandro Paes de Brito2, Francisca Crislândia Oliveira Silva2
1Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
Preparative biochemistry & biotechnology
|March 21, 2025
概括
这项研究使用乳酸细菌发酵从奶酪乳清中产生了外聚糖 (EPS). 优化生产产生了显著的EPS,具有显著的抗氧化特性,突出显示奶酪乳清作为可持续的生物聚合物来源.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 奶酪乳清 (CW) 是一种富含营养的副产品,具有生物技术应用的潜力.
- 使用CW减少了对环境的影响,并为微生物发酵提供了低成本的基材.
- 从CW生产增值化合物支持乳制品行业的循环经济.
研究的目的:
- 用于在奶酪乳糖中发酵的乳酸细菌产生外聚糖 (EPS).
- 使用响应表面方法 (RSM) 优化EPS生产.
- 描述产生的EPS的生物特性.
主要方法:
- 乳酸细菌在奶酪乳糖中的发酵.
- 使用RSM优化发酵参数 (温度,时间).
- 描述EPS成分和评估抗氧化活性 (DPPH,ABTS,超氧化物,基).
主要成果:
- * 球菌 (Enterococcus) * sp. 这种球菌. 133V产生了5.58毫克/毫升的EPS.
- 在优化条件下 (42°C,22小时) 产生了21.74 mg/mL EPS.
- EPS是一种具有抗氧化特性的异多多糖,对DPPH,ABTS和超氧化基具有显著的清除作用.
结论:
- 奶酪乳清是一种可行的和具有成本效益的基质,用于微生物外聚糖的生产.
- 优化发酵显著提高了EPS产量.
- 生产的EPS表现出有价值的生物特性,表明在食品和生物技术领域的潜在应用.
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