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烯硫酸键作为构建具有增强响应率的醇探针的一般支架

Tao Ma^1,2, Jintao Zhao², Bingbing Chang²

¹School of Chemistry and Chemical Engineering, Nanjing University of Science & Technology, Nanjing, Jiangsu 210094, China.

Analytical chemistry

|March 21, 2025

概括

这项研究引入了硫 (-Se-S-) 探针来检测醇,显示出比二硫化物探针更快的反应. 新的近红外探测器ASC-SeS揭示了药物诱导的肝损伤中的醇枯竭.

科学领域:

化学生物学化学生物学
生物化学生物化学
有机化学有机化学

背景情况:

二硫化物和二化物键是光探针检测细胞内还原性物种的有效触发器.
烯硫化物,与diselenides和disulfides之间的反应性,在探测器设计中未得到充分研究.

研究的目的:

为了比较二硫化物 (-S-S-),-硫 (-Se-S-) 和硫- (-S-Se-) 单位在醇探针中的效率.
为了开发一种多功能-Se-S-支架用于醇识别和设计近红外 (NIR) 光探测器,ASC-SeS.
研究硫醇检测的机制,并将探针应用于生物系统.

主要方法:

对探头结构中的 -S-S-, -Se-S-,和 -S-Se-结构单元进行比较分析.
设计和合成的NIR光探测器ASC-SeS结合 -Se-S-单位.
关于键断裂和循环化动态的机制研究.
在活细胞成像和体内研究中ASC-SeS的应用.

主要成果:

-Se-S脚手架展示了对硫醇的多功能识别.
探测器ASC-SeS显示,由于中间酸盐的循环转化速度更快,醇的反应速度加快.
在使用ASC-SeS.的药物诱导性肝损伤 (DILI) 模型中观察到严重的醇耗尽.

结论:

线性烯硫化物广泛适用于加速醇探针响应率.
与 -S-S-和 -S-Se-.相比, -Se-S-单元是醇检测的优越触发器.
ASC-SeS是可视化生物背景中的醇动态的宝贵工具,包括DILI等疾病状态.

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