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Updated: May 21, 2025

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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理性设计一种全新的多功能酶基生物传感器类别

Shihao Pei1, Sofie Dhondt1, Samuel Babity1

  • 1Faculté de Pharmacie, Université de Montréal, 2940 Chemin de Polytechnique, Montréal, Québec H3T 1J4, Canada.

Analytical chemistry
|March 21, 2025
PubMed
概括

这项研究引入了新的单分子生物传感器,通过将酶与pH敏感的光体结合起来,用于无接触式生物标志物检测. 这些光传感器能够进行定量分析,推动下一代生物传感平台的发展.

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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