暴露于卡巴马塞的地的特点是特定于组织的积累模式和转录形状
Chubin Zhang1, Filipe Cabreiro2, Leon P Barron3
1Analytical, Environmental & Forensic Sciences Department, Institute of Pharmaceutical Sciences, School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London SE1 9NH, United Kingdom.
Environment international
|March 21, 2025
概括
卡巴马兹是一种抗药物,会在暴露在污染土壤中的地中积累,改变其肠道微生物群,并引起组织特异性的分子变化. 这突显了制药污染对土壤健康的风险.
科学领域:
- 环境毒理学环境毒理学
- 生态毒理学 生态毒理学
- 土壤科学 土壤科学
背景情况:
- 药品,如卡巴马西平,通过废水再利用和生物固体应用污染土壤.
- 卡巴马泽的非特异性作用会影响非目标生物,包括土壤虫,这可能会影响土壤质量.
研究的目的:
- 为了研究地 (Dendrobaena veneta) 中的卡巴马西平吸收动态.
- 评估卡巴马西平对子分子和生命周期终点的影响.
- 了解土壤生态系统中新出现的污染物的生态毒理学影响.
主要方法:
- 在实验室条件下,长达28天的时间里,地暴露在不同度的碳胺 (0.6和10毫克/公斤) 的土壤中.
- 在土壤和地组织中,使用液态染色学双重质谱法 (LC-MS/MS) 量化了卡巴马泽的度.
- 分析了土壤和肠道微生物组的变化,以及地的基因表达 (de novo RNA测序).
主要成果:
- 在虫中,卡巴马西平的积累是取决于组织,剂量和时间的.
- 暴露调节的土壤和地肠道微生物群.
- 观察到组织特异性的转录组变化,包括改变的突蛋白聚合促进蛋白 (tppp) 表达,细胞色素P450反应有限.
结论:
- 卡巴马塞暴露会在陆地虫中产生显著的,组织特异性的分子影响.
- 虫使用的排毒途径与水生模型不同.
- 调查结果强调需要对新出现的污染物进行全面的生态毒理学评估,以告知与废水和生物固体实践相关的风险评估.
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