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软海 (Pelodiscus sinensis) 对饥饿的代谢反应,通过综合代谢组和转录组分析揭示出来
Xueying Shi1, Qingqing Guo1, Xiangce Li1
1Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
概括
软的海通过促进葡萄糖的生产和改变脂质和氨基酸代谢,维持饥饿期间的血糖. 这种适应性策略有助于它们在长时间的食物剥夺中生存.
科学领域:
- 比较生理学比较生理学
- 代谢生物化学 代谢生物化学
- 动物适应 动物适应
背景情况:
- 动物面临饥饿,但能量调动有所不同.
- 了解这些机制对于动物的生存至关重要.
- 软的海 (Pelodiscus sinensis) 作为一个模型生物体.
研究的目的:
- 在饥饿期间调查Pelodiscus sinensis的能量调动和代谢调整.
- 确定参与饥饿反应的关键基因,代谢物和途径.
- 阐明P. sinensis对食物剥夺的适应策略.
主要方法:
- 将八十只乌置于1,4,8,16和32天的饥饿期.
- 分析身体组成,肝脏葡萄糖原和血代谢物的变化.
- 使用比较转录学和代谢学来识别差异性基因和代谢物.
- 进行途径丰富分析以了解代谢转变.
主要成果:
- 身体成分显示水分损失最大,其次是蛋白质和脂质.
- 4天后,肝脏葡萄糖素显著降低并稳定.
- 血葡萄糖,胆固醇和自由脂肪酸从第8天开始下降,而三糖醇从第4天开始下降.
- 葡萄糖生成基因 (pepck,g6pase) 被上调,以保持葡萄糖平衡.
- 对比分析显示,32天和1天饥饿的海之间有6051个不同的基因和150个不同的代谢物.
- 已确定的主要代谢途径包括甘氨酸脂,氨酸/酸盐/酸盐和氨酸/低氨酸代谢.
- 观察到酸丁胆,氨酸和其他代谢物的水平增加,支持葡萄糖生成和细胞保护.
结论:
- 在饥饿期间,Pelodiscus sinensis通过上调葡萄糖生成来维持葡萄糖平衡.
- 激活脂质和氨基酸代谢是生存食物剥夺的关键适应策略.
- 特定的代谢物在支持葡萄糖生成,细胞膜完整性和减轻细胞损伤方面发挥着至关重要的作用.
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