乳酸脱酶和酸合成酶活性在女鱼和红雀鱼之间的物种间的差异
Elliott Schmidt1, Hunter Milles2, Lauren Kennedy3
1ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Australia; College of Science and Engineering, James Cook University, Townsville, Australia.
Journal of thermal biology
|March 21, 2025
概括
鱼的肌肉酶活性反映了物种之间的有氧和无氧呼吸差异. 鱼表现出更高的有氧酶活性,而红雀鱼表现出更高的无氧酶活性,与它们的活性水平相关.
科学领域:
- 比较生理学比较生理学
- 鱼类生物能源 鱼类生物能源
- 酶动力学 酶动力学
背景情况:
- 物种表现出对能源需求的多样化的生理适应,受热分布,生命史和行为的影响.
- 肌肉酶活性,特别是酸盐合成酶 (CS) 和乳酸脱酶 (LDH),分别作为有氧和无氧新陈代谢的代理.
- 以前的研究表明,酶活性的物种间差异与不同的代谢策略相关.
研究的目的:
- 为了比较鱼 (Pomacentrus) 和红雀鱼 (Ostorhinchus) 种类之间的CS和LDH最大酶活性.
- 研究温度对不同物种酶活性和热敏感性的影响.
- 将酶活性差异与特定物种的行为和代谢策略联系起来.
主要方法:
- 酸盐合成酶 (CS) 和乳酸脱酶 (LDH) 的最大酶活性在鱼和红雀鱼中被测量.
- 热性能曲线是通过在10°C间隔的10°C到50°C的温度下测试酶活性来生成的.
- 计算温度系数 (Q10) 来评估酶的热敏感性.
主要成果:
- CS和LDH都显示出与温度的正相关性,但没有达到平原.
- 鱼表现出更高的最大CS活性,表明有氧能力更大,而红雀鱼的LDH/CS比率更高,表明更多地依赖无氧代谢.
- 静止的红雀鱼物种Ostorhinchus doederleini表现出更高的最大LDH活性. 在Q10值中没有发现物种间的差异,这表明酶的热敏感性相似.
结论:
- 在最大酶活性的物种间差异支持代谢策略和生态利基之间的联系.
- 女鱼的较高的CS活动与持续游泳和积极运动的能量需求相一致.
- 红雀鱼中较高的LDH活性和较高的LDH/CS比率表明,它们依赖无氧代谢来进行冲浪游泳行为,特别是在久坐的物种中.
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