Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An antibiotic chatbot: Evaluation of a retrieval-augmented generation approach for providing guideline-based antimicrobial advice.

The Journal of infection·2026
Same author

Multicenter Validation of Clinical Sepsis Phenotypes.

JAMA network open·2026
Same author

Rapid diagnosis of common, undetected, and uncultivable bloodstream infections from positive blood cultures using Oxford Nanopore sequencing: a metagenomic pipeline analysis.

The Lancet. Microbe·2026
Same author

How ready are we to use artificial intelligence in our fight against antimicrobial resistance? An ESGAID and EAAS perspective.

Expert review of anti-infective therapy·2026
Same author

Safety and efficacy of CRS3123 in adults with a primary episode or first recurrence of Clostridioides difficile infection: a phase 2, randomised, double-blind, multicentre, vancomycin-controlled study.

The Lancet. Infectious diseases·2026
Same author

Influence of dental implant surfaces on oral biofilms and host immune response.

Journal of oral microbiology·2026

相关实验视频

Updated: May 12, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
11:13

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Published on: September 14, 2013

38.7K

复杂的Clostridioides difficile分子钟的复杂性.

Jon J Vernon1, David W Eyre2, Mark H Wilcox3

  • 1Healthcare-Associated Infections Research Group, Leeds Institute for Medical Research, University of Leeds, Wellcome Trust Brenner Building, St James University Hospital, West Yorkshire, Leeds, LS9 7TF, UK; Division of Oral Biology, School of Dentistry, University of Leeds, Wellcome Trust Brenner Building, St James University Hospital, West Yorkshire, Leeds, LS9 7TF, UK.

Anaerobe
|March 21, 2025
PubMed
概括

对于Clostridioides difficile进化的分子钟模型可能比预期的更复杂. 细菌子休眠期可能会影响植物遗传学分析和分歧率估计.

关键词:
抗微生物耐药性 抗微生物耐药性细菌进化的过程克洛斯特里迪奥伊德斯困难菌氨基氨基 (Fluoroquinolone) 是一种可以人类遗传学 是一个学科.带纹型为027 带纹型

更多相关视频

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

12.5K
A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

9.0K

相关实验视频

Last Updated: May 12, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
11:13

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Published on: September 14, 2013

38.7K
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

12.5K
A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

9.0K

科学领域:

  • 微生物学 微生物学
  • 进化生物学 进化生物学
  • 基因组学就是基因组学.

背景情况:

  • 困难菌 (C. diff) 是医疗保健相关感染的重要原因之一.
  • 了解C. diff的进化动态对于公共卫生干预至关重要.
  • 历史隔离物提供了对病原体演变的宝贵见解.

研究的目的:

  • 为了重建历史C. diff分离的遗传关系.
  • 评估分子时钟模型在估计C.diff分离率时的准确性.
  • 评估进化轨迹与既定时钟模型之间的一致性.

主要方法:

  • 使用Illumina测序对历史C.diff分离物 (1980-86) 的遗传学分析.
  • 与当代欧洲监测和遗传学数据的比较.
  • 应用再组合调整的最大概率树和分子时钟计算 (0.74个SNP/基因组/年).

主要成果:

  • 在历史隔离物中观察到的SNP分歧往往偏离了分子钟预测.
  • 一些孤立的SNP比预期的少,挑战了当前的差异率估计.
  • 根到尖的分析显示出弱的线性相关性,表明复杂的进化模式.

结论:

  • 对C. diff的分子钟估计可能需要改进.
  • 细菌子静止可能是复杂化遗传学和进化速率分析的一个因素.
  • 需要进一步的研究来了解C. diff进化的细微差别.