从Parageobacillus thermoglucosidasius中检测到的独特基因中推断跨系基因转移事件
A Cuecas1, J A Delgado2, J M Gonzalez1
1Institute of Natural Resources and Agrobiology of Seville - Spanish National Research Council (IRNAS-CSIC), Avda. Reina Mercedes 10, Seville 41012, Spain.
Molecular phylogenetics and evolution
|March 21, 2025
概括
横向基因转移 (HGT) 丰富了细菌基因组,赋予了适应性优势. 这项研究揭示了距离较远的细菌族之间经常发生的HGT事件,主要涉及从Bacillota族出口的DNA.
科学领域:
- 微生物基因组学和进化过程
- 横向的基因转移机制
- 细菌的遗传学学.
背景情况:
- 全基因组包括物种内的所有基因,包括横向获得的基因.
- 水平基因转移 (HGT) 提供了适应性优势,增强了微生物的功能和生长.
- 之前的研究表明,Bacillota系内经常发生DNA交换,但不太了解家族间的转移.
研究的目的:
- 通过使用Parageobacillus thermoglucosidasius的泛基因组来调查潜在的族群间水平基因转移 (HGT) 事件.
- 在P. thermoglucosidasius中识别独特的基因,并在遥远的细菌种群中寻找同源基因.
- 推断HGT事件中涉及的方向性和主导类.
主要方法:
- 对Parageobacillus thermoglucosidasius的泛基因组进行分析.
- 利用独特的基因对不同细菌种群进行BLAST搜索,以检测同源序列.
- 根据基因分布和频率推导潜在的族际HGT事件.
主要成果:
- 有证据表明,不同细菌类之间发生了基因交换,包括Actinomycetota,Pseudomonadota和Bacteroidota/Chlorobiota群.
- 经常转移的基因包括ATP结合盒载体,碳代谢酶和转录调节剂.
- 分析表明,HGT事件在Bacillota族中具有主导性起源,这表明它们是DNA的重要出口者.
结论:
- 对Parageobacillus thermoglucosidasius的泛基因组分析显示了广泛的族际HGT.
- 巴西洛塔属似乎是出口DNA的主要来源,无论是在属内还是属外.
- 了解远距离HGT对于理解微生物进化, prokaryotic基因组可塑性和HGT的意义至关重要.
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